Last Updated: June 25, 2026

Claims for Patent: 8,415,160

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Summary for Patent: 8,415,160

Title:	Transfection with micro explosion enacted by coated dry ice particles
Abstract:	Disclosed is a transfection method, which includes the steps of: (a) adhering the gene fragments to dry ice particles; (b) adding the dry ice particles into the medium/liquid that contains target cells; and (c) transporting the gene fragments into the target cells via the micro explosion/sublimation of the dry ice particles. In addition, the gene fragments can also adhere first to nanoparticles, which can then adhere to dry ice particles. Subsequently, gene fragments enter cells by micro explosion/sublimation. The present invention can be applied in transgenic research on prokaryotic, eukaryotic, plant and animal cells and in the development of new species in agriculture.
Inventor(s):	Fu-Jen Kao, Yung-En Kuo
Assignee:	National Yang Ming Chiao Tung University NYCU
Application Number:	US12/957,035

Details for Patent 8,415,160

Glossary

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Bluebird Bio Inc.	SKYSONA	elivaldogene autotemcel	Injection	125755	September 16, 2022	⤷ Start Trial	2030-11-30
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

International Patent Family for US Patent 8,415,160

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Country	Patent Number	Estimated Expiration
Taiwan	201204833	⤷ Start Trial
Taiwan	I385251	⤷ Start Trial
United States of America	2012028356	⤷ Start Trial
>Country	>Patent Number	>Estimated Expiration

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