Claims for Patent: 8,187,879

Summary for Patent: 8,187,879

Title:	Collection and selection methods to isolate a stem cell population from human adult periodontal follicular tissues
Abstract:	Methods for the isolation, expansion and storage of a population of stem cells belonging to human dental follicles, called FENC (Follicle-derived Embryonic Neural Crest stem cells,) including: a) Collection of the follicular sack in sterile conditions, digestion and primary culture growth and expansion; b) Optional amplification; c) FACsorting.
Inventor(s):	Laino; Gregorio (Torre del Greco, IT), Papaccio; Gianpaolo (Naples, IT), De Rosa; Alfredo (Naples, IT), D\'Aquino; Riccardo (Naples, IT), Graziano; Antonio (Naples, IT)
Assignee:	Teslab S.R.L. (cava de\' tirreni, unknown)
Application Number:	12/279,853
Patent Claims:	1. A method for the isolation of a population of stem cells from human dental follicles comprising: a) collecting intact human dental follicular sacks; b) digesting the follicular sacks in sterile conditions to produce a digestive solution; c) filtering and then centrifuging the digestive solution to produce a cellular suspension; d) isolating the cellular suspension; e) culturing the cellular suspension in a culture media specific for stem cells to produce a primary culture; f) optionally allowing the cells in the primary culture to amplify; g) isolating stem cells by FACsorting using antibodies against SSEA-4, TRA 1-60, TRA1-81, CD133, CD90, flk-1, and OCT-4. 2. The method according to claim 1, in which the digestion is carried out using proteolytic enzymes and antibiotics. 3. The method according to claim 2, in which the enzymatic digestion is carried out by means of an aqueous solution containing: 3 mg/mL type I collagenase, 4 mg/mL dispase, 100 U/mL penicillin, 100 .mu.g/mL streptomycin, 500 .mu.g/mL clarytromicin in PBS. 4. The method according to claim 3, in which the enzymatic digestion is carried out for 1 hour at 37.degree. C., under shaking. 5. The method according to claim 1, in which the culture media is supplemented with 10% p/v FBS, 100 .mu.M 2P-ascorbic acid, 2 mM L-glutamine, 100 U/mL penicillin, 100 .mu.g/mL streptomycin. 6. The method according to claim 5, in which the incubation is carried out at 37.degree. C. and in an atmosphere of 5% CO.sub.2 for 15-20 days, changing the medium every 3 days.

Details for Patent 8,187,879

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2026-02-20