Claims for Patent: 8,029,992

Summary for Patent: 8,029,992

Title:	Methods of evaluating cells and cell cultures
Abstract:	Methods of evaluating the composition of a cell culture (e.g., to distinguish chondrocytes from fibroblasts) and methods for evaluating the phenotype of an individual cell (e.g., as a chondrocyte) are disclosed. The methods may be used, for example, for assessing chondrocyte cultures used for treatment of cartilage defects. In some embodiments, the invention involves identifying cell culture composition or the identity of a cell based on expression level of a fibroblast marker. In other embodiments, the invention involves comparing expression levels of at least one chondrocyte marker and at least one fibroblast marker in a cell culture sample or in an individual cell. In illustrative embodiments, the chondrocyte marker is hyaluronan and proteoglycan link protein 1 (HAPLN1), and the fibroblast marker is microfibrillar associated protein 5 (MFAP5).
Inventor(s):	Stephen M. Rapko, Stephen J. Duguay
Assignee:	Vericel Corp
Application Number:	US12/098,033

Details for Patent 8,029,992

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Vericel Corporation	MACI	autologous cultured chondrocytes on porcine collagen membrane	Cell Sheets	125603	December 13, 2016	8,029,992	2028-04-04

International Patent Family for US Patent 8,029,992

Country	Patent Number	Estimated Expiration
World Intellectual Property Organization (WIPO)	2008124508
United States of America	2012329051
United States of America	2012149013