Claims for Patent: 6,056,957

Summary for Patent: 6,056,957

Title:	Humanized monoclonal antibodies against human interleukin-5
Abstract:	A monoclonal antibody is provided which specifically binds to human interleukin-5. Also provided are a hybridoma which produces the monoclonal antibody; complementary DNAs which encode the heavy and light chain variable regions of the monoclonal antibody and CDRs therefrom; humanized monoclonal antibodies; and pharmaceutical compositions comprising the monoclonal antibody or anti-idiotypic antibodies directed against it, humanized monoclonal antibodies, binding fragments, binding compositions or single-chain binding proteins derived from the antibody and a physiologically acceptable carrier.
Inventor(s):	Chou; Chuan-Chu (Westfield, NJ), Murgolo; Nicholas J. (Millington, NJ), Abrams; John S. (Belmont, CA), Jenh; Chung-Her (Edison, NJ), Petro; Mary E. (Green Pond, NJ), Silver; Jon E. (San Jose, CA), Tindall; Stephen (Madison, NJ), Windsor; William T. (East Brunswick, NJ), Zavodny; Paul J. (Mountainside, NJ)
Assignee:	Schering Corporation (Kenilworth, NJ)
Application Number:	08/284,516
Patent Claims:	1. A monoclonal antibody or fragment thereof which specifically binds to human interleukin-5 comprising a heavy chain variable region defined by SEQ ID NO: 1 and/or a light chain variable region defined by SEQ ID NO: 2. 2. A hybridoma secreting a monoclonal antibody which specifically binds human interleukin-5, said antibody having a heavy chain variable region defined by SEQ ID NO: 1 and/or a light chain variable region defined by SEQ ID NO: 2. 3. A binding composition capable of specifically binding to human interleukin-5 comprising a polypeptide comprising an amino acid sequence defined by SEQ ID NO: 1 or SEQ ID NO:2. 4. The binding composition of claim 3 wherein the amino acid sequence is defined by SEQ ID NO: 1. 5. The binding composition of claim 3 wherein the amino acid sequence is defined by SEQ ID NO: 2. 6. An isolated DNA which comprises a nucleotide sequence encoding a polypeptide comprising an amino acid sequence defined by SEQ ID NO: 1 or 2. 7. The DNA of claim 6 which encodes a polypeptide comprising the amino acid sequence defined by SEQ ID NO: 1. 8. The DNA of claim 7 comprising the nucleotide sequence defined by SEQ ID NO: 1. 9. The DNA of claim 6 which encodes a polypeptide comprising the amino acid sequence defined by SEQ ID NO: 2. 10. The DNA of claim 9 comprising the nucleotide sequence defined by SEQ ID NO: 2. 11. A recombinant vector comprising the DNA of claim 6. 12. A host cell comprising the recombinant vector of claim 11. 13. A method of making a polypeptide comprising culturing the host cell of claim 12 under conditions in which the DNA is expressed. 14. A humanized monoclonal antibody or fragment thereof which specifically binds to human interleukin-5 and comprises complementarity determining regions from the heavy and light chain variable regions of the monoclonal antibody of claim 1. 15. The humanized monoclonal antibody of claim 14 comprising a heavy or light chain variable region comprising a mature amino acid sequence set forth in SEQ ID NO: 36, 37, 40, 41, 46, 47, 56, 57, 64 or 65. 16. The humanized monoclonal antibody of claim 14 comprising a heavy chain variable region comprising the mature amino acid sequence defined in SEQ ID NO: 36 and a light chain variable region comprising the mature amino acid sequence set forth in SEQ ID NO: 37. 17. The humanized monoclonal antibody of claim 14 comprising a heavy chain variable region comprising the mature amino acid sequence defined in SEQ ID NO: 40 and a light chain variable region comprising the mature amino acid sequence set forth in SEQ ID NO: 41. 18. The humanized monoclonal antibody of claim 14 comprising a heavy chain variable region comprising the mature amino acid sequence defined in SEQ ID NO: 46 and a light chain variable region comprising the mature amino acid sequence set forth in SEQ ID NO: 47. 19. The humanized monoclonal antibody of claim 14 comprising a heavy chain variable region comprising the mature amino acid sequence defined in SEQ ID NO: 56 and a light chain variable region comprising the mature amino acid sequence set forth in SEQ ID NO: 57. 20. The humanized monoclonal antibody of claim 14 comprising a heavy chain variable region comprising the mature amino acid sequence defined in SEQ ID NO: 64 and a light chain variable region comprising the mature amino acid sequence set forth in SEQ ID NO: 65. 21. A binding composition capable of specifically binding to human interleukin-5 comprising a polypeptide comprising a variable region of a humanized monoclonal antibody which comprises a mature amino acid sequence defined by SEQ ID NO: 36, 37, 40, 41, 46, 47, 56, 57, 64 or 65. 22. An isolated DNA which comprises a nucleotide sequence encoding a mature amino acid sequence defined by SEQ ID NO: 36, 37, 40, 41, 46, 47, 56, 57, 64 or 65. 23. A recombinant vector comprising the DNA of claim 22. 24. A host cell comprising the recombinant vector of claim 23. 25. A method of making a polypeptide comprising culturing the host cell of claim 24 under conditions in which the DNA is expressed. 26. A pharmaceutical composition comprising the humanized monoclonal antibody of claim 14 and a pharmaceutically acceptable carrier. 27. An IL-5 binding protein comprising complementary determining regions from the heavy and/or light chain variable regions of the monoclonal antibody of claim 1. 28. The IL-5 binding protein of claim 27 which is a single chain IL-5 binding protein. 29. The binding composition of claim 3, wherein the polypeptide which comprises an amino acid sequence defined by SEQ ID NO: 1 and SEQ ID NO: 2. 30. The DNA of claim 6 which encodes an amino acid sequence defined by SEQ ID NO: 1 and SEQ ID NO: 2.

Details for Patent 6,056,957

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Teva Respiratory, Llc	CINQAIR	reslizumab	Injection	761033	03/23/2016		2017-05-02