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Last Updated: April 19, 2024

Claims for Patent: 5,948,413

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Summary for Patent: 5,948,413

Title:	Method and vaccine for treatment of pythiosis insidiosi in humans and lower animals
Abstract:	A method and vaccine for treatment of pythiosis in humans and animals is described. In particular a vaccine comprising a mixture of extracellular and intracellular proteins is described. The vaccine enables cures of chronic pythiosis in some patients.
Inventor(s):	Mendoza; Alberto L. (Haslett, MI)
Assignee:	Board of Trustees operating Michigan State University (East Lansing, MI)
Application Number:	08/895,940
Patent Claims:	1. A method for providing an injectable vaccine for treatment of Pythiosis which comprises: (a) growing cells of Pythium insidiosum in a culture medium; (b) separating the cells from a first supernatant of the culture medium which contains extracellular proteins; (c) killing the cells; (d) disrupting the cells in sterile water; (e) separating the disrupted cells from the water to produce a second supernatant containing intracellular proteins; (f) mixing the first supernatant of step (b) with the second supernatant of step (e); (g) separating the combined proteins from the mixture of step (f); (h) mixing the separated proteins in sterile distilled water; and (i) dialyzing the mixture of step (h) to remove low molecular weight components less than 10,000 MW to produce the vaccine. 2. The method of claim 1 wherein the cells are disrupted by sonication. 3. The method of claim 1 wherein the Pythium insidiosum is deposited as ATCC 74446. 4. The method of any one of claims 1, 2 or 3 wherein the culture medium is Sabouraud's dextrose broth. 5. The method of claim 1 wherein the cells are killed with thimersol. 6. The method of claim 1 wherein the disrupted cells are separated from the water in step (e) by centrifugation. 7. The method of claim 1 wherein the separated proteins are separated in step (g) by being precipitated together using acetone from the first and second supernatants combined together.

Details for Patent 5,948,413

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006	⤷ Try a Trial	2039-09-23
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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