Claims for Patent: 5,614,399

Summary for Patent: 5,614,399

Title:	Plant ubiquitin promoter system
Abstract:	A method of inducibly enhancing the constitutive expression of a DNA sequence of interest is described in which plant cells are transformed with a DNA sequence of interest that is operably joined to a plant ubiquitin regulatory region comprised of a heat shock element, a promoter, a transcription start site, an intron, and a translation start site. When monocot or dicot plant cells are subjected to permissive heat shock temperatures, the level of expression of the DNA sequence of interest is enhanced.
Inventor(s):	Quail; Peter H. (Richmond, CA), Christensen; Alan H. (Albany, CA), Hershey; Howard P. (West Chester, PA), Sharrock; Robert A. (El Cerrito, CA), Sullivan; Thomas D. (Madsion, WI)
Assignee:	Mycogen Plant Science, Inc. (San Diego, CA)
Application Number:	08/462,092
Patent Claims:	1. A method for selective heat shock induced enhancement of the constitutive expression of a structural gene in a plant cell comprising the steps of: (a) transforming said plant cell with a DNA construct comprising an approximately 2 kb plant ubiquitin regulatory region operably joined to a DNA sequence of interest, wherein said plant ubiquitin regulatory region is from a plant ubiquitin gene and comprises at least one heat shock element, a promoter, a transcription start site, and an intron; and (b) selectively applying stress conditions of high temperature to said transformed plant cell thereby inducing enhancement in expression of said DNA sequence of interest. 2. A method as in claim 1 wherein said regulatory region comprises two heat shock elements. 3. A method as in claim 2 wherein said heat shock elements are situated so that the elements overlap. 4. A method as in claim 1 wherein said intron is approximately 1 kb in length. 5. A method as in claim 1 wherein said plant ubiquitin regulatory region is from a maize ubiquitin gene. 6. A method as in claim 1 wherein said plant cell is a monocot plant cell. 7. A method as in claim 1 wherein said plant cell is a dicot plant cell. 8. A method as in claim 6 wherein said monocot plant is oat. 9. A method as in claim 7 wherein said dicot plant is tobacco. 10. A method as in claim 1 wherein said DNA sequence of interest encodes a structural protein.

Details for Patent 5,614,399

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26