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Last Updated: January 26, 2022

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Claims for Patent: 5,385,832

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Summary for Patent: 5,385,832
Title: Method for obtaining highly pure beta 1,3-glucan from euglena
Abstract:Substantially pure, pyrogen-free beta-1,3-glucan is produced by cultivating Euglena cells in a defined growth medium and under specified conditions that provide a cell mass comprising 70% to 90% beta-1,3-glucan on a dry weight basis, separating the cell mass from the supernatant, extracting the cell mass with methanol and chloroform, acid-washing the extracted cell mass, and washing the acid-treated material with water.
Inventor(s): Tuse; Daniel (Fremont, CA), Marquez; Leticia (Berkeley, CA), Hokama; Leslie A. (Mountain View, CA)
Assignee: SRI International (Menlo Park, CA)
Application Number:08/034,609
Patent Claims:1. A process for obtaining substantially pure beta-1,3-glucan from a culture of Euglena cells in which the cells comprise about 70% to 90% beta-1,3-glucan on a dry weight basis comprising carrying out the following steps under aseptic conditions:

(a) separating the cells from the culture;

(b) lysing the cells and extracting the cells with an extractant that removes lipid and pigment from the cells and obtaining crystalline beta-1,3-glucan;

(c) separating the crystalline beta-1,3-glucan from the extractant;

(d) adding acid to the crystalline beta-1,3-glucan with heating and agitation to form an acid soluble and an acid insoluble portion, said acid insoluble portion containing the crystalline beta-1,3-glucan; and

(e) washing the acid insoluble portion containing the crystalline beta-1,3-glucan with sterile water to produce said substantially pure beta-1,3-glucan of at least about 95% purity containing less than about 0.08 endotoxin units per milliliter.

2. The process of claim 1 wherein the extractant comprises methanol and chloroform.

3. The process of claim 1 wherein the acid is dilute HCl.

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