Claims for Patent: 10,412,941

Summary for Patent: 10,412,941

Title:	Artificial feeding method at low altitude for host insect ghost moth of ophiocordyceps sinensis
Abstract:	An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis including selecting an appropriate disinfectant for performing surface disinfection on ghost moth eggs, and then placing the disinfected eggs in sterile humus; feeding ghost moth larvae with carrots, disinfecting and detecting the fed carrots, and culturing the larvae till male and female pupae are obtained; identifying male and female pupae of the ghost moths, placing them at different culture temperatures, wherein the culture temperature for the male pupae is 2-6.degree. C. lower than that of the female pupae, resulting synchronously emerged male and female adults; placing the emerged male and female adults in an adult cage with simulated plants for mating and staying of the adults, maintaining a humidity over 80%, and employing 50-100 Lux shimmer for facilitating activity and mating of the adults.
Inventor(s):	Cao; Li (Guangzhou, CN), Han; Richou (Guangzhou, CN)
Assignee:	GUANGDONG INSTITUTE OF APPLIED BIOLOGICAL RESOURCES (Guanzhou, Guangdong, CN)
Application Number:	15/117,330
Patent Claims:	1. An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis, comprising the following steps: (a). managing an egg stage by performing surface disinfection on a first egg laid by ghost moth adults to kill bacteria and control fungi to form a disinfected egg and, then placing the disinfected egg in sterile humus; (b). managing a larva stage by feeding ghost moth larvae with carrots, wherein the step (b) comprises washing the carrots with sterile water and aqueous ethanol with volume fraction of 75%, dicing the carrots and then sampling a portion of the carrots by feeding the ghost moth larvae for at least two days to detect whether the carrots have a lethal effect on larvae, and preserving a remaining portion of the carrots at low temperature for later use, wherein if the portion of the carrots sampled does not cause harm to larvae, the remaining portion of the carrots preserved at low temperature can be used for feeding larvae to obtain male pupae and female pupae; (c). managing a pupa stage by identifying the male pupae and the female pupae of ghost moths, placing the male pupae and the female pupae at different culture temperatures, wherein the culture temperature of the male pupae is 2-6.degree. C. lower than that of the female pupae, such that development of the male pupae becomes slow and is synchronous with that of the female pupae; and (d). managing an adult stage by placing male adults and female adults that have emerged from the pupa stage (c) in an adult cage in which simulated plants are placed for mating and staying of the male adults and the female adults, maintaining a humidity over 80%, employing 50-100 Lux shimmer for facilitating activity and mating of the male adults and the female adults, and collecting a second egg after the female adults lay the second egg. 2. The artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis according to claim 1, wherein surface disinfection in step (a) comprises washing the first egg with sterile water for several times and then disinfecting with a disinfectant for 3-6 minutes, filtering out and then washing with sterile water for several times again, the disinfectant is prepared by adding 150 ml 4 mol NaOH solution, 50 ml sodium hypochlorite (the content of active chlorine .gtoreq.7.5%) to per 1000 ml sterile water, and the humus contains 35-45% of water.

Details for Patent 10,412,941

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006		2034-08-20