Claims for Patent: 10,031,080

Summary for Patent: 10,031,080

Title:	Method for recognizing resistant germs and device for performing same
Abstract:	A method for detecting resistant germs in a sample includes contacting the sample by a sample carrier which has an agent for killing different germs. In addition, the method includes introducing the sample carrier into an analyzer and detecting light emissions from the sample carrier by the analyzer. An indication is output by the analyzer indicating that the sample contains at least one germ resistant to the agent for killing different germs, if the light emission exceeds a threshold value, or indicating that the sample does not contain germs resistant to the agent for killing different germs, if the light emission does not exceed the threshold value.
Inventor(s):	Schmidt; Jennifer (Falkenstein, DE), Eisele; Ignaz (Icking, DE), Trupp; Sabine (Saalfeld, DE), Haberger; Karl (Martinsried, DE), Sittel; Wolfgang (Bad Homburg, DE)
Assignee:	Fraunhofer-Gesellschaft zur Forderung der angewandten Forschung e.V. (DE) Asklepios Kliniken Verwaltungsgesellschaft mbH (DE)
Application Number:	15/172,386
Patent Claims:	1. A method for detecting living multi-resistant germs in a sample, comprising: contacting the sample by a sample carrier which comprises an agent for killing different germs; wherein the agent comprises a combination of antibiotics and/or antimyotics; introducing the sample carrier in an analyzer; detecting light emissions of the living multi-resistant germs from the sample carrier by the analyzer; wherein the light emissions result from enzymatic activity of proteins comprising DNAse and/or catalase and/or hyaluronidase formed by pathogens still living; outputting an indication by the analyzer indicating that the sample comprises at least one multi-resistant germ resistant to the agent for killing different germs, if the light emission exceeds a threshold value and the at least one multi-resistant germ is thus identified in a germ-unspecific manner; and outputting an indication by the analyzer indicating that the sample does not comprise living multi-resistant germs resistant to the agent for killing different germs, if the light emission does not exceed the threshold value. 2. The method in accordance with claim 1, wherein the sample carrier for contacting the sample by the sample carrier additionally comprises a nutrient for different germs. 3. The method in accordance with claim 1, further comprising: providing a colorant which marks living germs in the sample carrier or renders a metabolic product of the living germs in the sample carrier measurable, wherein providing the colorant takes place such that, in contacting the colorant by the sample, the colorant is split based on a splitting process of the colorant induced by the metabolic product of the germs and the colorant is activated; and contacting the colorant by the sample. 4. The method in accordance with claim 3, wherein the sample carrier for contacting the sample by the sample carrier comprises the colorant. 5. The method in accordance with claim 1, further comprising: providing a photomultiplier or a single-photon detector for detecting the light emission; illuminating the sample carrier by electromagnetic radiation on which light emission from the sample carrier is based. 6. The method in accordance with claim 1, further comprising: evaluating a kind or strain of the living multi-resistant germ resistant to the agent for killing different germs; outputting an indication by the analyzer indicating the kind or strain of the living multi-resistant germ. 7. The method in accordance with claim 1, further comprising: heating the sample carrier to a temperature in a range between 35.degree. C. and 40.degree. C. after introducing the sample carrier in the analyzer. 8. The method in accordance with claim 1, further comprising: shaking the sample carrier when the sample carrier is contacted by the sample. 9. A device for detecting living multi-resistant germs in a sample, comprising: a sample carrier which comprises an agent for killing different germs, wherein the agent comprises a combination of antibiotics and/or antimyotics; and an analyzer configured to detect light emissions of the living multi-resistant germs resulting from enzymatic activity of proteins comprising DNAse and/or catalase and/or hyaluronidase formed by pathogens still living; wherein the analyzer is configured to output an indication indicating that the sample comprises at least one multi-resistant germ resistant to the agent for killing different germs, if the light emission exceeds a threshold value and multi-resistant germs are thus identified in a germ-unspecific manner; and to output an indication indicating that the sample does not comprise multi-resistant germs resistant to the agent for killing different germs, if the light emission does not exceed the threshold value. 10. The device in accordance with claim 9, further comprising a container configured to comprise a colorant and release same to the sample carrier. 11. The device in accordance with claim 9, wherein the analyzer comprises a photomultiplier or a single-photon detector configured to detect light emissions from the sample carrier. 12. The device in accordance with claim 9, further comprising an illuminator configured to illuminate the sample carrier. 13. The device in accordance with claim 9, wherein the analyzer is further configured to determine a kind or strain of the living multi-resistant germ resistant to the agent for killing different germs and to output an indication indicating the kind or strain of the living multi-resistant germ. 14. The device in accordance with claim 9, further comprising a heating element configured to heat the sample carrier to a temperature in a range between 35.degree. C. and 40.degree. C. 15. The device in accordance with claim 9, further comprising a shaker for shaking the sample carrier.

Details for Patent 10,031,080

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Bausch & Lomb Incorporated	VITRASE	hyaluronidase	Injection	021640	May 05, 2004	10,031,080	2036-06-03
Bausch & Lomb Incorporated	VITRASE	hyaluronidase	Injection	021640	December 02, 2004	10,031,080	2036-06-03
Amphastar Pharmaceuticals, Inc.	AMPHADASE	hyaluronidase	Injection	021665	October 26, 2004	10,031,080	2036-06-03
Akorn, Inc.	HYDASE	hyaluronidase	Injection	021716	October 25, 2005	10,031,080	2036-06-03