Details for Patent: 5,854,026

Title:	Human growth hormone variant having enhanced affinity for human growth hormone receptor at site 1
Abstract:	A human growth hormone variant including the set of amino acid substitutions H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A is disclosed. Also disclosed is a nucleic acid encoding this variant, along with a vector including the nucleic acid, a host cell including the vector, and a process for preparing the variant. The variant has enhanced affinity for human growth hormone receptor at site 1.
Inventor(s):	Cunningham; Brian C. (Piedmont, CA), Wells; James A. (Burlingame, CA)
Assignee:	Genentech, Inc. (South San Francisco, CA)
Filing Date:	Jun 07, 1995
Application Number:	08/487,110
Claims:	1. An isolated variant of a naturally occurring human growth hormone having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions: H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A. 2. The variant of claim 1 wherein said variant is covalently attached to a polyethylene glycol molecule. 3. An isolated nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions: H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A. 4. A vector comprising a nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions: H18A, Q22A, F26A, D26A, Q29A, E65A, K168A, E174A. 5. A host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions: H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A. 6. A process for preparing a human growth hormone variant comprising: (a) culturing a host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant having a greater affinity for human growth hormone receptor at site 1 than said naturally occurring human growth hormone, said variant comprising the following set of amino acid substitutions: H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A; and (b) recovering the variant from the culture.