Details for Patent: 8,334,296

Title:	2,4-pyrimidinediamine compounds and their uses
Abstract:	The present invention provides 2,4-pyrimidinediamine compounds that inhibit the IgE and/or IgG receptor signaling cascades that lead to the release of chemical mediators, intermediates and methods of synthesizing the compounds and methods of using the compounds in a variety of contexts, including in the treatment and prevention of diseases characterized by, caused by or associated with the release of chemical mediators via degranulation and other processes effected by activation of the IgE and/or IgG receptor signaling cascades.
Inventor(s):	Singh; Rajinder (Belmont, CA), Argade; Ankush (Foster City, CA), Payan; Donald G (Hillsborough, CA), Molineaux; Susan (San Mateo, CA), Holland; Sasha J (San Francisco, CA), Clough; Jeffrey (Redwood City, CA), Keim; Holger (Irvine, CA), Bhamidipati; Somasekhar (Foster City, CA), Sylvain; Catherine (Burlingame, CA), Li; Hui (Santa Clara, CA), Rossi; Alexander B (Reedsport, OR)
Assignee:	Rigel Pharmaceuticals, Inc. (South San Francisco, CA)
Filing Date:	Nov 18, 2008
Application Number:	12/273,357
Claims:	1. A method of treating a disease characterized by, caused by or associated with mast or basophil cell degranulation, wherein the disease is an allergic disease, the method comprising administering to an animal suffering from such a disease an effective amount of a compound according to formula I: ##STR00065## or in the form of a pharmaceutically acceptable salt thereof, wherein: each of R.sup.2 and R.sup.4, independently is phenyl substituted with one or more R.sup.8 groups or a heteroaryl selected from the group consisting of ##STR00066## ##STR00067## wherein at least one of R.sup.2 and R.sup.4 is the heteroaryl; R.sup.2 is not trialkoxyphenyl; R.sup.5 is selected from the group consisting of halo, (C1-C6) alkyl optionally substituted with one or more of the same or different R.sup.8 groups, --OR.sup.d, --SR.sup.d, NR.sup.cR.sup.c, (C1-C3) haloalkyl, --CN, --NO.sub.2, --C(O)R.sup.d, --C(O)OR.sup.d, --C(O)NR.sup.cR.sup.c, --C(NH)NR.sup.cR.sup.c, --OC(O)R.sup.d, --SC(O)R.sup.d, --OC(O)OR.sup.d, --SC(O)OR.sup.d, --OC(O)NR.sup.cR.sup.c; --SC(O)NR.sup.cR.sup.c, --OC(NH)NR.sup.cR.sup.c, --SC(NH)NR.sup.cR.sup.c, --[NHC(O)].sub.nR.sup.d, --[NHC(O)].sub.nOR.sup.d, --[NHC(O)].sub.nNR.sup.cR.sup.c, --[NHC(NH)].sub.nNR.sup.cR.sup.C, (C5-C10) aryl optionally substituted with one or more of the same or different R.sup.8 groups, and 5-10 membered heteroaryl optionally substituted with one or more of the same or different R.sup.8 groups; R.sup.35 is hydrogen; each R.sup.8 is independently R.sup.e, R.sup.b, R.sup.e substituted with one or more of the same or different R.sup.a or R.sup.b, --OR.sup.a substituted with one or more of the same or different R.sup.a or R.sup.b, --B(OR.sup.a).sub.2, --B(NR.sup.cR.sup.c).sub.2, --(CH.sub.2).sub.m--R.sup.b, --(CHR.sup.a).sub.m--R.sup.b, --O--(CH.sub.2).sub.m--R.sup.b, --S--(CH.sub.2).sub.m--R.sup.b, --O--CHR.sup.aR.sup.b, --O--CR.sup.a(R.sup.b).sub.2, --O--(CHR.sup.a).sub.m--R.sup.b, --O--(CH.sub.2).sub.m--CH[(CH.sub.2).sub.mR.sup.b]R.sup.b, --S--(CHR.sup.a).sub.m--R.sup.b, --C(O)NH--(CH.sub.2).sub.m--R.sup.b, --C(O)NH--(CHR.sup.a).sub.m--R.sup.b, --O--(CH.sub.2).sub.m--C(O)NH--(CH.sub.2).sub.m--R.sup.b, --S--(CH.sub.2).sub.m--C(O)NH--(CH.sub.2).sub.m--R.sup.b, --O--(CHR.sup.a).sub.m--C(O)NH--(CHR.sup.a).sub.m--R.sup.b, --S--(CHR.sup.a).sub.m--C(O)NH--(CHR.sup.a).sub.m--R.sup.b, --NH--(CH.sub.2).sub.m--R.sup.b, --NH--(CHR.sup.a).sub.m--R.sup.b, --NH[(CH.sub.2).sub.mR.sup.b], --N[(CH.sub.2).sub.mR.sup.b].sub.2, --NH--C(O)--NH--(CH.sub.2).sub.m--R.sup.b, --NH--C(O)--(CH.sub.2).sub.m--CHR.sup.bR.sup.b or --NH--(CH.sub.2).sub.m--C(O)--NH--(CH.sub.2).sub.m--R.sup.b; each R.sup.a is independently selected from the group consisting of hydrogen, (C1-C6) alkyl, (C3-C8) cycloalkyl, (C4-C11) cycloalkylalkyl, (C5-C10) aryl, (C6-C16) arylalkyl, 2-6 membered heteroalkyl, 3-8 membered cycloheteroalkyl, 4-11 membered cycloheteroalkylalkyl, 5-10 membered heteroaryl and 6-16 membered heteroarylalkyl; each R.sup.b is a suitable group independently selected from the group consisting of --OR.sup.d, (C1-C3) haloalkyloxy, --OCF.sub.3, --SR.sup.d, --NR.sup.cR.sup.c, halogen, --CF.sub.3, --CN, --NC, --OCN, --SCN, --NO, --NO.sub.2, .dbd.N.sub.2, --N.sub.3, --S(O)R.sup.d, --S(O).sub.2R.sup.d, --S(O).sub.2OR.sup.d, --S(O)NR.sup.cR.sup.c, --S(O).sub.2NR.sup.cR.sup.c, --OS(O)R.sup.d, --OS(O).sub.2R.sup.d, --OS(O).sub.2OR.sup.d, --OS(O).sub.2NR.sup.cR.sup.c, --C(O)R.sup.d, --C(O)OR.sup.d, --C(O)NR.sup.cR.sup.c, --C(NH)NR.sup.cR.sup.c, --C(NR.sup.a)NR.sup.cR.sup.c, --C(NOH)R.sup.a, --C(NOH)NR.sup.cR.sup.c, --OC(O)R.sup.d, --OC(O)OR.sup.d, --OC(O)NR.sup.cR.sup.c, --OC(NH)NR.sup.cR.sup.c, --OC(NR.sup.a)NR.sup.cR.sup.c, --[NHC(O)].sub.nR.sup.d, --[NR.sup.aC(O)].sub.nR.sup.d, --[NHC(O)].sub.nOR.sup.d, --[NR.sup.aC(O)].sub.nOR.sup.d, --[NHC(O)].sub.nNR.sup.cR.sup.c, --[NR.sup.aC(O)].sub.nNR.sup.cR.sup.c, --[NHC(NH)].sub.nNR.sup.cR.sup.c and --[NR.sup.aC(NR.sup.a)].sub.nNR.sup.cR.sup.c; each R.sup.c is independently R.sup.a; each R.sup.d is independently R.sup.a; each R.sup.e is independently selected from the group consisting of (C1-C6) alkyl, (C3-C8) cycloalkyl, (C4-C11) cycloalkylalkyl, (C5-C10) aryl, (C6-C16) arylalkyl, 2-6 membered heteroalkyl, 3-8 membered cycloheteroalkyl, 4-11 membered cycloheteroalkylalkyl, 5-10 membered heteroaryl and 6-16 membered heteroarylalkyl; each Y.sup.1 independently is selected from the group consisting of O, S, SO, SO.sub.2, SONR.sup.36, NH and NR.sup.35; each Y.sup.2 independently is selected from the group consisting of CH, CH.sub.2, O, S, N, NH and NR.sup.35; each R.sup.36 independently is hydrogen or alkyl; A is selected from the group consisting of O, NH and NR.sup.38; Q is selected from the group consisting of --OH, OR.sup.8, --NR.sup.cR.sup.c, NHR.sup.39C(O)R.sup.8, --NHR.sup.39--C(O)OR.sup.8, --NR.sup.39--CHR.sup.40--R.sup.b, --NR.sup.39--(CH.sub.2).sub.m--R.sup.b and --NR.sup.39--C(O)--CHR.sup.40--NR.sup.cR.sup.c; each R.sup.38 independently is selected from the group consisting of alkyl and aryl; R.sup.9, R.sup.10, R.sup.11 and R.sup.12 are each, independently of one another, selected from the group consisting of alkyl, alkoxy, halogen, haloalkoxy, aminoalkyl and hydroxyalkyl, or, alternatively, R.sup.9 and R.sup.10 or R.sup.11 and R.sup.12, or R.sup.9 and R.sup.10 and R.sup.11 and R.sup.12 are taken together form an oxo group; each Z is selected from the group consisting of hydroxyl, alkoxy, aryloxy, ester and carbamate; R.sup.39 and R.sup.40 are each, independently of one another, selected from the group consisting of hydrogen, alkyl, aryl, alkylaryl, arylalkyl and NHR.sup.8; each m independently is an integer from 1 to 3; and each n independently is an integer from 0 to 3. 2. The method of claim 1 in which the animal is a human. 3. A method of treating a disease mediated at least in part by Syk kinase activity, wherein the disease is an allergic disease, the method comprising the step of administering to an animal in need thereof an amount of a 2,4-pyrimidinediamine compound effective to inhibit the Syk kinase activity, thereby treating the disease, wherein the compound is according to formula I: ##STR00068## or in the form of a pharmaceutically acceptable salt thereof, wherein: each of R.sup.2 and R.sup.4, independently is phenyl substituted with one or more R.sup.8 groups or a heteroaryl selected from the group consisting of ##STR00069## ##STR00070## wherein at least one of R.sup.2 and R.sup.4 is the heteroaryl; R.sup.2 is not trialkoxyphenyl; R.sup.5 is selected from the group consisting of halo, (C1-C6) alkyl optionally substituted with one or more of the same or different R.sup.8 groups, --OR.sup.d, --SR.sup.d, --NR.sup.cR.sup.c, (C1-C3) haloalkyl, --CN, --NO.sub.2, --C(O)R.sup.d, --C(O)OR.sup.d, --C(O)NR.sup.cR.sup.c, --C(NH)NR.sup.cR.sup.c, --OC(O)R.sup.d, --SC(O)R.sup.d, --OC(O)OR.sup.d, --SC(O)OR.sup.d, --OC(O)NR.sup.cR.sup.c; --SC(O)NR.sup.cR.sup.c, --OC(NH)NR.sup.cR.sup.c, --SC(NH)NR.sup.cR.sup.c, --[NHC(O)].sub.nR.sup.d, --[NHC(O)].sub.nOR.sup.d, --[NHC(O)].sub.nNR.sup.cR.sup.c, --[NHC(NH)].sub.nNR.sup.cR.sup.c, (C5-C10) aryl optionally substituted with one or more of the same or different R.sup.8 groups, and 5-10 membered heteroaryl optionally substituted with one or more of the same or different R.sup.8 groups; R.sup.35 is hydrogen; each R.sup.8 is independently R.sup.e, R.sup.b, R.sup.e substituted with one or more of the same or different R.sup.a or R.sup.b, --OR.sup.a substituted with one or more of the same or different R.sup.a or R.sup.b, --B(OR.sup.a).sub.2, --B(NR.sup.cR.sup.c).sub.2, --(CH.sub.2).sub.m--R.sup.b, --(CHR.sup.a).sub.m--R.sup.b, --O--(CH.sub.2).sub.m--R.sup.b, --S--(CH.sub.2).sub.m--R.sup.b, --O--CHR.sup.aR.sup.b, --O--CR.sup.a(R.sup.b).sub.2, --O--(CHR.sup.a).sub.m--R.sup.b, --O--(CH.sub.2).sub.m--CH[(CH.sub.2).sub.mR.sup.b]R.sup.b, --S--(CHR.sup.a).sub.m--R.sup.b, --C(O)NH--(CH.sub.2).sub.m--R.sup.b, --C(O)NH--(CHR.sup.a).sub.m--R.sup.b, --O--(CH.sub.2).sub.m--C(O)NH--(CH.sub.2).sub.m--R.sup.b, --S--(CH.sub.2).sub.m--C(O)NH--(CH.sub.2).sub.m--R.sup.b, --O--(CHR.sup.a).sub.m--C(O)NH--(CHR.sup.a).sub.m--R.sup.b, --S--(CHR.sup.a).sub.m--C(O)NH--(CHR.sup.a).sub.m--R.sup.b, --NH--(CH.sub.2).sub.m--R.sup.b, --NH--(CHR.sup.a).sub.m--R.sup.b, --NH[(CH.sub.2).sub.mR.sup.b], --N[(CH.sub.2).sub.mR.sup.b].sub.2, --NH--C(O)--NH--(CH.sub.2).sub.m--R.sup.b, --NH--C(O)--(CH.sub.2).sub.m--CHR.sup.bR.sup.b or --NH--(CH.sub.2).sub.m--C(O)--NH--(CH.sub.2).sub.m--R.sup.b; each R.sup.a is independently selected from the group consisting of hydrogen, (C1-C6) alkyl, (C3-C8) cycloalkyl, (C4-C11) cycloalkylalkyl, (C5-C10) aryl, (C6-C16) arylalkyl, 2-6 membered heteroalkyl, 3-8 membered cycloheteroalkyl, 4-11 membered cycloheteroalkylalkyl, 5-10 membered heteroaryl and 6-16 membered heteroarylalkyl; each R.sup.b is a suitable group independently selected from the group consisting of --OR.sup.d, (C1-C3) haloalkyloxy, --OCF.sub.3, --SR.sup.d, --NR.sup.cR.sup.c, halogen, --CF.sub.3, --CN, --NC, --OCN, --SCN, --NO, --NO.sub.2, .dbd.N.sub.2, --N.sub.3, --S(O)R.sup.d, --S(O).sub.2R.sup.d, --S(O).sub.2OR.sup.d, --S(O)NR.sup.cR.sup.c, --S(O).sub.2NR.sup.cR.sup.c, --OS(O)R.sup.d, --OS(O).sub.2R.sup.d, --OS(O).sub.2OR.sup.d, --OS(O).sub.2NR.sup.cR.sup.c, --C(O)R.sup.d, --C(O)OR.sup.d, --C(O)NR.sup.cR.sup.c, --C(NH)NR.sup.cR.sup.c, --C(NR.sup.a)NR.sup.cR.sup.c, --C(NOH)R.sup.a, --C(NOH)NR.sup.cR.sup.c, --OC(O)R.sup.d, --OC(O)OR.sup.d, --OC(O)NR.sup.cR.sup.c, --OC(NH)NR.sup.cR.sup.c, --OC(NR.sup.a)NR.sup.cR.sup.c, --[NHC(O)].sub.nR.sup.d, --[NR.sup.aC(O)].sub.nR.sup.d, --[NHC(O)].sub.nOR.sup.d, --[NR.sup.aC(O)].sub.nOR.sup.d, --[NHC(O)].sub.nNR.sup.cR.sup.c, --[NR.sup.aC(O)].sub.nNR.sup.cR.sup.c, --[NHC(NH)].sub.nNR.sup.cR.sup.c and --[NR.sup.aC(NR.sup.a)].sub.nNR.sup.cR.sup.c; each R.sup.c is independently R.sup.a; each R.sup.d is independently R.sup.a; each R.sup.e is independently selected from the group consisting of (C1-C6) alkyl, (C3-C8) cycloalkyl, (C4-C11) cycloalkylalkyl, (C5-C10) aryl, (C6-C16) arylalkyl, 2-6 membered heteroalkyl, 3-8 membered cycloheteroalkyl, 4-11 membered cycloheteroalkylalkyl, 5-10 membered heteroaryl and 6-16 membered heteroarylalkyl; each Y.sup.1 independently is selected from the group consisting of O, S, SO, SO.sub.2, SONR.sup.36, NH and NR.sup.35; each Y.sup.2 independently is selected from the group consisting of CH, CH.sub.2, O, S, N, NH and NR.sup.35; each R.sup.36 independently is hydrogen or alkyl; A is selected from the group consisting of O, NH and NR.sup.38; Q is selected from the group consisting of --OH, OR.sup.8, --NR.sup.cR.sup.c, NHR.sup.39C(O)R.sup.8, --NHR.sup.39--C(O)OR.sup.8, --NR.sup.39--CHR.sup.40--R.sup.b, --NR.sup.39--(CH.sub.2).sub.m--R.sup.b and --NR.sup.39--C(O)--CHR.sup.40--NR.sup.cR.sup.c; each R.sup.38 independently is selected from the group consisting of alkyl and aryl; R.sup.9, R.sup.10, R.sup.11 and R.sup.12 are each, independently of one another, selected from the group consisting of alkyl, alkoxy, halogen, haloalkoxy, aminoalkyl and hydroxyalkyl, or, alternatively, R.sup.9 and R.sup.10 or R.sup.11 and R.sup.12, or R.sup.9 and R.sup.10 and R.sup.11 and R.sup.12 are taken together form an oxo group; each Z is selected from the group consisting of hydroxyl, alkoxy, aryloxy, ester and carbamate; R.sup.39 and R.sup.40 are each, independently of one another, selected from the group consisting of hydrogen, alkyl, aryl, alkylaryl, arylalkyl and NHR.sup.8; each m independently is an integer from 1 to 3; and each n independently is an integer from 0 to 3. 4. The method of claim 3 in which the animal is a human. 5. The method of claim 1 wherein the disease is asthma.