Details for Patent: 8,067,569

Title:	Splice-region antisense composition and method
Abstract:	Antisense compositions targeted against an mRNA sequence coding for a selected protein, at a region having its 5' end from 1 to about 25 base pairs downstream of a normal splice acceptor junction in the preprocessed mRNA, are disclosed. The antisense compound is RNase-inactive, and is a phosphorodiamidate-linked morpholino oligonucleotide containing uncharged phosphorodiamidate linkages interspersed with cationic phosphorodiamidate linkages. Such targeting is effective to inhibit natural mRNA splice processing, produce splice variant mRNAs, and inhibit normal expression of the protein.
Inventor(s):	Iversen; Patrick L. (Corvallis, OR), Hudziak; Robert (Blodgett, OR), Weller; Dwight D. (Corvallis, OR)
Assignee:	AVI BioPharma, Inc. (Corvallis, OR)
Filing Date:	May 11, 2006
Application Number:	11/433,214
Claims:	1. An antisense morpholino oligomer containing between 12 and 40 morpholino subunits, wherein said subunits are (a1) linked together by either uncharged or positively charged, piperazine-containing phosphorodiamidate linkages, such that said uncharged phosphorodiamidate linkages are interspersed with at least two and up to half positively charged, piperazine-containing phosphorodiamidate linkages, and (a2) support a sequence of purine or pyrimidine base-pairing moieties, including a sequence between 12 and 25 nucleotide bases in length that is complementary to a target region of a selected preprocessed mRNA coding for a selected protein, where the 5' end of the target region is 1-25 bases downstream of a normal splice acceptor site in said preprocessed mRNA, and having the properties that: (b1): the oligomer is taken up by eukaryotic cells; (b2): the oligomer hybridizes to the target region of preprocessed mRNA in such cells but does not hybridize to a splice acceptor junction, and (b3): the oligomer so hybridized to the target pre-mRNA prevents splicing at said normal acceptor splice site, such that the splice mechanism proceeds to a downstream splice acceptor site in the pre-mRNA, producing a splice variant processed mRNA with a truncated coding sequence. 2. The oligomer of claim 1, wherein said morpholino subunits are linked by said phosphorodiamidate linkages in accordance with the structure: ##STR00002## where Y.sub.1.dbd.O, Z.dbd.O, Pj is a purine or pyrimidine base-pairing moiety effective to bind, by base-specific hydrogen bonding, to a base in a polynucleotide, and X for the uncharged linkages is an amine of the form NR.sub.2, where each R is independently hydrogen or methyl, and X for the positively charged linkages is 1-piperazine. 3. The oligomer of claim 2, wherein the 5' end of the target region is 2-20 bases downstream of said normal splice acceptor site. 4. The oligomer of claim 3, wherein the 5' end of the target region is 2-15 bases downstream of said normal splice acceptor site. 5. The oligomer of claim 2, wherein said downstream splice acceptor site is a whole multiple of three bases downstream of the normal splice acceptor site, such that said splice variant mRNA has a coding sequence in frame with that of the processed mRNA when it is normally spliced. 6. The oligomer of claim 2, wherein said selected protein has multiple distinct binding regions, and said truncated coding sequence codes for a variant protein in which one such binding region is disabled. 7. The oligomer of claim 1, wherein said truncated coding sequence codes for a dominant negative protein. 8. The oligomer of claim 7, wherein said selected protein is human c-myc, and said variant protein is an N-terminal truncated c-myc. 9. The oligomer of claim 8, having a base sequence selected from the group consisting of SEQ ID NO: 3 and SEQ ID NOs: 13 through 28. 10. The oligomer of claim 1, wherein the selected protein and corresponding antisense base sequence is selected from the group consisting of (a) human chorionic gonadotropin, .beta. subunit: SEQ ID NO: 6; (b) human androgen receptor: SEQ ID NO: 9 or 12; (c) human c-myc: SEQ ID NO: 3, 13, 29 or 30; (d) human p53: SEQ ID NO: 35; (e) human abl: SEQ ID NO: 31; and (0 HIV-1 rev: SEQ ID NO: 33. 11. A method of inhibiting normal splicing of mRNA in a eukaryotic cell, comprising contacting the cell with an antisense morpholino oligomer containing between 12 and 40 morpholino subunits, wherein said morpholino subunits are (a1) linked together by either uncharged or positively charged, piperazine-containing phosphorodiamidate linkages, such that said uncharged phosphorodiamidate linkages are interspersed with at least two and up to half positively charged, piperazine-containing phosphorodiamidate linkages, and (a2) support a sequence of purine or pyrimidine base-pairing moieties, including a sequence between 12 and 25 nucleotide bases in length that is complementary to a target region of a selected preprocessed mRNA coding for a selected protein, where the 5' end of the target region is 1-25 bases downstream of the a normal splice acceptor site in said preprocessed mRNA, wherein the oligomer: (b1): is taken up by the cell; (b2): hybridizes to the target region of preprocessed mRNA in the cell but does not hybridize to a splice acceptor junction, and (b3): being so hybridized, (i) prevents splicing at said normal acceptor splice site, such that (ii) the splice mechanism proceeds to a downstream splice acceptor sequence in the mRNA, producing a splice variant processed mRNA with a truncated coding sequence. 12. The method of claim 11, wherein said morpholino subunits are joined by phosphorodiamidate linkages, in accordance with the structure: ##STR00003## where Y.sub.1.dbd.O, Z.dbd.O, Pj is a purine or pyrimidine base-pairing moiety effective to bind, by base-specific hydrogen bonding, to a base in a polynucleotide, and X for the uncharged linkages is an amine of the form wherein NR.sub.2, where each R is independently hydrogen or methyl, and X for the positively charged linkages is 1-piperazine. 13. The method of claim 12, wherein the 5' end of the target region is 2-20 bases downstream of said normal splice acceptor site. 14. The compound of claim 13, wherein the 5' end of the target region is 2-15 bases downstream of said normal splice acceptor site. 15. The method of claim 12, wherein said downstream splice acceptor site is a whole multiple of three bases downstream of the normal splice acceptor site, such that said splice variant mRNA has a coding sequence in frame with that of the processed mRNA when it is normally spliced. 16. The method of claim 11, wherein the selected protein has multiple distinct binding regions, and said truncated coding sequence codes for a variant protein in which a binding region is disabled. 17. The method of claim 16, wherein said variant protein is a dominant negative protein. 18. The method of claim 17, wherein said selected protein is human c-myc, and said variant protein is an N-terminal truncated c-myc. 19. The method of claim 18, wherein the antisense oligomer has a base sequence selected from the group consisting of SEQ ID NO: 3 and SEQ ID NOs: 13 through 28. 20. The method of claim 11, wherein the selected protein and corresponding antisense base sequence is selected from the group consisting of (a) human chorionic gonadotropin, .beta. subunit: SEQ ID NO: 6; (b) human androgen receptor: SEQ ID NO: 9 or 12; (c) human c-myc: SEQ ID NO: 3, 13, 29, or 30; (d) human p53: SEQ ID NO: 35; (e) human abl: SEQ ID NO: 31; and (f) HIV-1 rev: SEQ ID NO: 33.