|Title:||Multiple antigen glycopeptide carbohydrate vaccine comprising the same and use thereof|
|Abstract:||A carbohydrate peptide conjugate containing: (i) a carrier containing a dendrimeric poly-lysine enabling multiple epitopes to be covalently attached thereto, (ii) at least one peptide containing one T epitope or several identical or different T-epitopes, (iii) at least one carbohydrate moiety which is tumor antigen, or a derivative thereof, containing a B epitope, provided it is not a sialoside, or several identical or different epitopes, wherein said conjugate containing at least 3-lysines and up to 15 lysine covalently linked to one another, and wherein: (a) to the NH.sub.2 and of at least two lysine residues is bound at least one carbohydrate residue being not a sialoside, optionally substituted and containing an epitope and wherein the peptide containing one T epitope is covalently bound to the end of said carbohydrate which induces immune responses.|
|Inventor(s):||Bay; Sylvie (Paris, FR), Cantacuzene; Daniele (Paris, FR), Leclerc; Claude (Paris, FR), Lo-Man; Richard (Paris, FR), Vicher-Guerre; Sophie (La Celle Saint Cloud, FR)|
|Assignee:||Institut Pasteur (FR)|
|Filing Date:||Sep 27, 1999|
|Claims:||1. A carbohydrate peptide conjugate selected from the group consisting of the conjugates of the following formulae (a) to (f): ##STR2## |
wherein: K is a lysyl residue, T is a CD4 T.sup.+ cell peptide epitope; B and Tn is a Tn antigen selected from the group consiting of .alpha.-Gal-Nac, .alpha.-Gal-Nac-Ser, .alpha.-Gal-Nac-Thr, .beta.-Gal-Nac, .beta.-Gal-Nac-Ser, .beta.-Gal-Nac-Thr, .beta.-Gal-(1-3)-.alpha.-Gal-Nac-Ser, .beta.-Gal(1-3).alpha.-Gal-Nac-Thr(.alpha.-Gal-Nac-Ser/Thr).sub.2, (.alpha.-Gal-Nac-Ser/Thr).sub.3 and (.alpha.-Gal-Nac-Ser/Thr).sub.6.
2. A immunogenic composition comprising the conjugate of claim 1 and a suitable carrier.
3. A immunogenic composition the conjugate of claim 1 and a suitable carrier and adjuvant.