|Title:||Enzymatically active recombinant glucocerebrosidase|
|Abstract:||Recombinant active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.|
|Inventor(s):||Rasmussen; James (Boston, MA), Barsomian; Gary (Georgetown, MA), Bergh; Michel (Belmont, MA)|
|Assignee:||Genzyme Corporation (Cambridge, MA)|
|Filing Date:||May 17, 1995|
|Claims:||1. A CHO cell comprising nucleic acid encoding enzymatically active human glucocerebrosidase, said cell being transformed with any plasmid selected from the group pGB20, pGB37 and pGB42.|
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