|Title:||High affinity TGf.beta. nucleic acid ligands and inhibitors|
|Abstract:||Methods are described for the identification and preparation of high-affinity nucleic acid ligands to TGF.beta.2. Included in the invention are specific RNA ligands to TGF.beta.2 identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of TGF.beta.2 with its receptor.|
|Inventor(s):||Pagratis; Nikos (Boulder, CO), Lochrie; Michael (Louisville, CO), Gold; Larry (Boulder, CO)|
|Assignee:||Gilead Sciences, Inc. (Foster City, CA)|
|Filing Date:||Jul 29, 1999|
|Claims:||1. A purified and isolated non-naturally occurring RNA ligand to transforming growth factor .beta.2 (TGF.beta.2) wherein said ligand is selected from the group consisting of the sequences set forth in Tables 5, 7, 8, 11, 13, 14, 16-19, and FIG. 9 (SEQ ID NOS: 21-121 and 128-193). |
2. The purified and isolated non-naturally occurring RNA ligand of claim 1 comprising SEQ ID NO:115 conjugated to polyethylene glycol (PEG), having the following structure: ##STR1##
3. The purified and isolated non-naturally occurring RNA ligand of claim 2 wherein said PEG has a molecular weight of about between 10-80 K.
4. The purified and isolated non-naturally occurring RNA ligand of claim 2 wherein said PEG has a molecular weight of about 20-45 K.
5. The purified and isolated non-naturally occurring RNA ligand of claim 2, wherein said ligand is ##STR2##
(SEQ ID NO:115), wherein rG is 2'OH G, rA is 2'OH A, fU is 2'F U and fC is 2'F C.
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.