|Title:|| Technetium-99m labeled peptides for imaging|
|Abstract:||This invention relates to radiolabeled peptides and methods for producing such peptides. Specifically, the invention relates to peptides, methods and kits for making such peptides, and methods for using such peptides to image sites in a mammalian body labeled with technetium-99m (Tc-99m) via Tc-99m binding moieties. In particular, the peptide reagents of the invention are covalently linked to a polyvalent linker moiety, so that the polyvalent linker moiety is covalently linked to a multiplicity of the specific-binding peptides, and the Tc-99m binding moieties are covalently linked to a plurality of the specific-binding peptides, the polyvalent linker moiety, or to both the specific-binding peptides and the polyvalent linker moiety.|
|Inventor(s):|| Dean; Richard T. (Bedford, NH), Lister-James; John (Bedford, NH) |
|Assignee:|| Diatide, Inc. (Londonderry, NH) |
|Filing Date:||Jun 06, 1995|
|Claims:||1. A kit comprising a sealed vial containing: |
a) a predetermined quantity of a reagent selected from the group consisting of:
(acetyl-F.sub.D PRPG).sub.2 KGGGCamide;
(GPRVVERHQSA).sub.2 KC.sub.Acm GC.sub.Acm amide; ##STR8## (CC.sub.Acm GC.sub.Acm GGRGDS).sub.3 -TSEA; (GPRPC.sub.Acm GC.sub.Acm Camide).sub.3 -TSEA;
(Pic.SC.sub.Acm SYNRGDSTCamide).sub.3 -TSEA;
(RALVDTLKGGC.sub.Acm GC.sub.Acm Camide).sub.3 -TSEA;
(GRGDFC.sub.Acm GC.sub.Acm Camide).sub.3 -TSEA;
(Pic.GC.sub.Acm RALVDTLKFVTQAETGAKCamide).sub.3 -TSEA; and
(acetyl-SYNRGDTC.sub.Acm GC.sub.Acm Camide).sub.2 -DMAB;
b) a sufficient amount of a reducing agent to label the reagent with technetium-99m.
2. A kit comprising a sealed vial containing a predetermined quantity of a reagent having a formula: ##STR9## and a sufficient amount of a reducing agent to label said reagent with technetium-99m.