Details for Patent: 5,962,314

Title:	Calcium receptor-active molecules
Abstract:	The present invention relates to the different roles inorganic ion receptors have in cellular and body processes. The present invention features: (1) molecules which can modulate one or more inorganic ion receptor activities, preferably the molecule can mimic or block an effect of an extracellular ion on a cell having an inorganic ion receptor, more preferably the extracellular ion is Ca.sup.2+ and the effect is on a cell having a calcium receptor; (2) inorganic ion receptor proteins and fragments thereof, preferably calcium receptor proteins and fragments thereof; (3) nucleic acids encoding inorganic ion receptor proteins and fragments thereof, preferably calcium receptor proteins and fragments thereof; (4) antibodies and fragments thereof, targeted to inorganic ion receptor proteins, preferably calcium receptor protein; and (5) uses of such molecules, proteins, nucleic acids and antibodies.
Inventor(s):	Brown; Edward M. (Milton, MA), Hebert; Steven C. (Wellesley, MA), Garrett, Jr.; James E. (Salt Lake City, UT)
Assignee:	NPS Pharmaceuticals, Inc. (Salt Lake City, UT) Brigham and Women's Hospital (Boston, MA)
Filing Date:	Oct 03, 1997
Application Number:	08/943,986
Claims:	1. An expression vector comprising at least 20 contiguous nucleotides of a nucleic acid sequence from nucleotide 515 to 3769 of SEQ ID NO: 1. 2. An expression vector comprising at least 27 contiguous nucleotides of a nucleic acid sequence from nucleotide 515 to 3769 of SEQ ID NO: 1. 3. An expression vector comprising at least 45 contiguous nucleotides of a nucleic acid sequence from nucleotide 515 to 3769 of SEQ ID NO: 1. 4. An expression vector comprising a nucleic acid sequence selected from the group consisting of: nucleotides 515 to 3769 of SEQ ID NO: 1, nucleotides 436 to 3699 of SEQ ID NO: 2, nucleotides 373 to 3606 of SEQ ID NO: 3 and nucleotides 574 to 3810 of SEQ ID NO: 4. 5. The expression vector of claim 4, wherein said nucleic acid sequence is SEQ ID NO: 1. 6. The expression vector of claim 4, wherein said nucleic acid sequence is SEQ ID NO: 2. 7. The expression vector of claim 4, wherein said nucleic acid sequence is SEQ ID NO: 3. 8. The expression vector of claim 4, wherein said nucleic acid sequence is SEQ ID NO: 4. 9. An expression vector comprising a nucleic acid sequence encoding at least 12 contiguous amino acids of an amino acid sequence provided in SEQ ID NO: 5. 10. An expression vector comprising a nucleic acid sequence encoding at least 18 contiguous amino acids of an amino acid sequence provided in SEQ ID NO: 5. 11. An expression vector comprising a nucleic acid sequence encoding at least 54 contiguous amino acids of an amino acid sequence provided in SEQ ID NO: 5. 12. An expression vector comprising a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8. 13. The expression vector of claim 12, wherein said amino acid sequence is SEQ ID NO: 5. 14. The expression vector of claim 12, wherein said amino acid sequence is SEQ ID NO: 6. 15. The expression vector of claim 12, wherein said amino acid sequence is SEQ ID NO: 7. 16. The expression vector of claim 12, wherein said amino acid sequence is SEQ ID NO: 8. 17. A recombinant cell comprising the expression vector of any one of claims 1-16. 18. A recombinant cell made by a process comprising the step of introducing the expression vector of any one of claims 1-16 into the cell. 19. A recombinant nucleic acid comprising at least 20 contiguous nucleotides of a sequence from nucleotide 515 to 3769 of SEQ ID NO: 1, transcriptionally coupled to an exogenous promoter. 20. A recombinant nucleic acid comprising at least 27 contiguous nucleotides of a sequence from nucleotide 515 to 3769 of SEQ ID NO: 1, transcriptionally coupled to an exogenous promoter. 21. A recombinant nucleic acid comprising at least 45 contiguous nucleotides of a sequence from nucleotide 515 to 3769 of SEQ ID NO: 1, transcriptionally coupled to an exogenous promoter. 22. A recombinant nucleic acid comprising a nucleic acid sequence selected from the group consisting of: nucleotides 515 to 3769 of SEQ ID NO: 1, nucleotides 436 to 3699 of SEQ ID NO: 2, nucleotides 373 to 3606 of SEQ ID NO: 3 and nucleotides 574 to 3810 of SEQ ID NO: 4, wherein said nucleic acid sequence is transcriptionally coupled to an exogenous promoter. 23. The recombinant nucleic acid of claim 22, wherein said nucleic acid sequence is SEQ ID NO: 1. 24. The recombinant nucleic acid of claim 22, wherein said nucleic acid sequence is SEQ ID NO: 2. 25. The recombinant nucleic acid of claim 22, wherein said nucleic acid sequence is SEQ ID NO: 3. 26. The recombinant nucleic acid of claim 22, wherein said nucleic acid sequence is SEQ ID NO: 4. 27. A recombinant nucleic acid comprising a nucleic acid sequence encoding at least 12 contiguous amino acids of an amino acid sequence provided in SEQ ID NO: 5, wherein said nucleic acid sequence is trancriptionally coupled to an exogenous promoter. 28. A recombinant nucleic acid comprising a nucleic acid sequence encoding at least 18 contiguous amino acids of an amino acid sequence provided in SEQ ID NO: 5, wherein said nucleic acid sequence is transcriptionally coupled to an exogenous promoter. 29. A recombinant nucleic acid comprising a nucleic acid sequence encoding at least 54 contiguous amino acids of an amino acid sequence provided in SEQ ID NO: 5, wherein said nucleic acid sequence is transcriptionally coupled to an exogenous promoter. 30. A recombinant nucleic acid comprising a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, wherein said nucleic acid sequence is transcriptionally coupled to an exogenous promoter. 31. The recombinant nucleic acid of claim 30, wherein said amino acid sequence is SEQ ID NO: 5. 32. The recombinant nucleic acid of claim 30, wherein said amino acid sequence is SEQ ID NO: 6. 33. The recombinant nucleic acid of claim 30, wherein said amino acid sequence is SEQ ID NO: 7. 34. The recombinant nucleic acid of claim 3, wherein said amino acid sequence is SEQ ID NO: 8. 35. A recombinant cell comprising the recombinant nucleic acid of any one of claims 19-34. 36. A recombinant cell made by a process comprising the step of introducing the recombinant nucleic acid of any one of claims 19-34 into the cell.