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Details for Patent: 5,879,658

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Details for Patent: 5,879,658

Title: Technetium-99m labeled peptides for thrombus imaging
Abstract:This invention relates to radiolabeled peptides and methods for producing such peptides. Specifically, the invention relates to specific binding peptides, methods and kits for making such peptides, and methods for using such peptides labeled with technetium-99m via a radiolabel-binding moiety covalently linked to the peptide to image thrombus sites in a mammalian body.
Inventor(s): Dean; Richard T. (Bedford, NH), Lister-James; John (Bedford, NH)
Assignee: Diatide, Inc. (Londonderry, NH)
Filing Date:Jun 07, 1995
Application Number:08/475,041
Claims:1. A reagent for preparing a thrombus imaging agent comprising:

a) a peptide, having an amino acid sequence of 4 to 100 amino acids, which specifically binds to a component of a thrombus; and

b) covalently linked to the peptide, a technetium-99m binding moiety selected from the group consisting of:

i) a technetium-99m binding moiety comprising a single thiol moiety having a formula:

wherein

A is H, HOOC, H.sub.2 NOC, or --NHOC;

B is SH or NHR";

X is H, methyl, SH or NHR";

Z is H or methyl;

R and R' are independently H or lower alkyl;

R" is H, lower alkyl or --C.dbd.O;

n is 0, 1 or 2;

and where B is NHR", X is SH, Z is H and n is 1 or 2;

where X is NHR", B is SH, Z is H and n is 1 or 2;

where B is H, A is HOOC, H.sub.2 NOC, or --NHOC, X is SH, Z is

H and n is 0or 1;

where Z is methyl, X is methyl, A is HOOC, H.sub.2 NOC, or

NHOC, B is SH and n is 0;

and wherein the thiol moiety is in the reduced form: ##STR17## wherein X=H or a protecting group; and (amino acid)=any amino acid; ##STR18## wherein X=H or a protecting group; and (amino acid)=any amino acid; ##STR19## wherein each R is independently H, CH.sub.3 or C.sub.2 H.sub.5 ;

each (pgp).sup.s is independently a thiol protecting group or H;

m, n and p are independently 2 or 3;

A=linear or cyclic lower alkyl, aryl, heterocyclyl,

a combination thereof or a substituted

derivative thereof; and ##STR20## wherein each R is independently H, CH.sub.3 or C.sub.2 H.sub.5 ;

m, n and p are independently 2 or 3;

A=linear or cyclic lower alkyl, aryl, heterocyclyl a combination thereof or a substituted derivative thereof;

V=H or --CO-peptide;

R'=H or peptide;

and wherein when V=H, R'=peptide and when R'=H, V=--CO-peptide.

2. The reagent according to claim 1 wherein the peptide and the technetium-99m binding moiety are covalently linked through one or more amino acids.

3. A composition of matter comprising the reagent according to claim 1 and a stannous ion.

4. A kit for preparing a radiopharmaceutical preparation, said kit comprising a sealed vial containing a predetermined quantity of a reagent according to claim 1 and a sufficient amount of a reducing agent to label said reagent with technetium-99m.

5. The reagent according to claim 1 wherein the peptide is chemically synthesized in vitro.

6. The reagent according to claim 5 wherein the peptide is synthesized by solid phase peptide synthesis.

7. The reagent according to claim 5 wherein the technetium-99m binding moiety is covalently linked to the peptide during in vitro chemical synthesis.

8. The reagent according to claim 7 wherein the technetium-99m binding moiety is covalently linked to the peptide during solid phase peptide synthesis.

9. A reagent comprising:

a) a peptide selected from the group consisting of:

KRARGDDMDDY,

GGGRGDF,

GGRGDF,

RGD,

GRGDGG,

GRGDGGGG,

RGDF,

NP.Apc.GD, and

GGGF.sub.D PRPGGGGNGDFEEIPEEYL; and

b) covalently linked to the peptide, a technetium-99m binding moiety comprising a single thiol moiety having a formula:

wherein

A is H, HOOC, H.sub.2 NOC, or --NHOC;

B is SH or NHR";

X is H, methyl, SH or NHR";

Z is H or methyl;

R and R' are independently H or lower alkyl;

R" is H, lower alkyl or --C.dbd.O;

n is 0, 1 or 2;

and where B is NHR", X is SH, Z is H and n is 1 or 2;

where X is NHR", B is SH, Z is H and n is 1 or 2;

where B is H, A is HOOC, H.sub.2 NOC, or --NHOC, X is SH, Z is H and n is 0 or 1;

where Z is methyl, X is methyl, A is HOOC, H.sub.2 NOC, or --NHOC, B is SH and n is 0;

and wherein the thiol moiety is in the reduced form.

10. A composition of matter comprising a reagent comprising:

a) a peptide selected from the group consisting of: ##STR21## NP.Apc.GD, ((GPRP).sub.2 K).sub.2 K,

(GPRVVERHQSA).sub.2 K,

GGF.sub.D PRPGGGGNGDFEEIPEEYL, and

GGGF.sub.D PRPGGGGNGDFEEIPEEYL; and

b) covalently linked to the peptide, a technetium-99m binding moiety comprising a single thiol moiety having a formula:

wherein

A is H, HOOC, H.sub.2 NOC, or --NHOC;

B is SH or NHR";

X is H, methyl, SH or NHR";

Z is H or methyl;

R and R' are independently H or lower alkyl;

R" is H, lower alkyl or --C.dbd.O;

n is 0, 1 or 2;

and where B is NHR", X is SH, Z is H and n is 1 or 2;

where X is NHR", B is SH, Z is H and n is 1 or 2;

where B is H, A is HOOC, H.sub.2 NOC, or --NHOC, X is SH, Z is H and n is 0 or 1;

where Z is methyl, X is methyl, A is HOOC, H.sub.2 NOC, or --NHOC, B is SH and n is 0;

and wherein the thiol moiety is in the reduced form.

11. A reagent comprising:

a) a peptide selected from the group consisting of: ##STR22## (SYNRGDSTC(S-maleimido)CH.sub.2 CH.sub.2 -).sub.3 N, and G.Apc.GDV.Apc.GDFKamide; and

b) covalently linked to the peptide, a technetium-99m binding moiety selected from the group consisting of: ##STR23## wherein X=H or a protecting group; and (amino acid)=any amino acid; ##STR24## wherein X=H or a protecting group; and (amino acid)=any amino acid; ##STR25## wherein each R is independently H, CH.sub.3 or C.sub.2 H.sub.5 ;

each (pgp).sup.s is independently a thiol protecting group or H;

m, n and p are independently 2 or 3;

A=linear or cyclic lower alkyl, aryl, heterocyclyl, a combination thereof or a substituted derivative thereof; and ##STR26## wherein each R is independently H, CH.sub.3 or C.sub.2 H.sub.5 ;

m, n and p are independently 2 or 3;

A=linear or cyclic lower alkyl, aryl, heterocyclyl, a combination thereof or a substituted derivative thereof;

V=H or --CO-peptide;

R'=H or peptide;

and wherein when V=H, R'=peptide and when R'=H, V=--CO-peptide.

12. A composition of matter comprising a reagent comprising:

a) a peptide selected from the group consisting of: ##STR27## ((GPRP).sub.2 K).sub.2 K, (GPRVVERHQSA).sub.2 K,

F.sub.D PRPGGGGNGDFEEIPEEYL,

GGGF.sub.D PRPGGGGNGDFEEIPEEYL, and

GGF.sub.D PRPGGGGNGDFEEIPEEYL; and

b) covalently linked to the peptide, a technetium-99m binding moiety selected from the group consisting of ##STR28## wherein X=H or a protecting group; and (amino acid)=any amino acid; ##STR29## wherein X=H or a protecting group; and (amino acid)=any amino acid; ##STR30## wherein each R is independently H, CH.sub.3 or C.sub.2 H.sub.5 ;

each (pgp).sup.s is independently a thiol protecting group or H;

m, n and p are independently 2 or 3;

A=linear or cyclic lower alkyl aryl, heterocyclyl, a combination thereof or a substituted derivative thereof; and ##STR31## wherein each R is independently H, CH.sub.3 or C.sub.2 H.sub.5 ;

m, n and p are independently 2 or 3;

A=linear or cyclic lower alkyl, aryl, heterocyclyl, a combination thereof or a substituted derivative thereof;

V=H or --CO-peptide;

R'=H or peptide;

and wherein when V=H, R'=peptide and when R'=H, V=--CO-peptide.
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