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Details for Patent: 5,859,228

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Details for Patent: 5,859,228

Title: Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
Abstract:This invention discloses a method for preparing a complex comprised of a VEGF Nucleic Acid Ligand and a Lipophilic Compound by identifying a VEGF Nucleic Acid Ligand by SELEX methodology and associating the VEGF Nucleic Acid Ligand with a Lipophilic Compound. The invention further discloses Complexes comprising one or more VEGF Nucleic Acid Ligands in association with a Lipophilic Compound. The invention further includes a Lipid construct comprising a VEGF Nucleic Acid Ligand or Complex and methods for making the same.
Inventor(s): Janjic; Nebojsa (Boulder, CO), Gold; Larry (Boulder, CO), Schmidt; Paul (Niwot, CO)
Assignee: NeXstar Pharmaceuticals, Inc. (Boulder, CO)
Filing Date:Oct 25, 1996
Application Number:08/739,109
Claims:1. A method for the preparation of a Complex comprised of a vascular endothelial growth factor (VEGF) Nucleic Acid Ligand and a Lipophilic Compound, said method comprising:

a) contacting the Candidate Mixture with VEGF, wherein Nucleic Acids having an increased affinity to VEGF relative to the Candidate Mixture may be partitioned from the remainder of the Candidate Mixture;

b) partitioning the increased affinity VEGF Nucleic Acids from the remainder of the Candidate Mixture;

c) amplifying the increased affinity VEGF Nuclcic Acids to yield a ligand-enriched mixture of Nucleic Acids; and

d) covalently linking said identified VEGF Nucleic Acid Ligand with a Lipophilic Compound.

2. The method of claim 1 wherein said Lipophilic Compound is a lipid.

3. The method of claim 2 wherein said lipid is selected from the group consisting of dialkylglycerol and diacyiglycerol.

4. The method of claim 1 wherein said Complex is further associated with a Lipid Construct.

5. The method of claim 4 wherein said Lipid Construct is a Lipid Bilayer Vesicle.

6. The method of claim 5 wherein said Lipid Bilayer Vesicle is a Liposome.

7. A method for preparing a Lipid Construct comprising a Complex comprised of a VEGF Nucleic Acid Ligand and a Lipophilic Compound, said method comprising:

a) contacting the Candidate Mixture with VEGF, wherein Nucleic Acids having an increased affinity to VEGF relative to the Candidate Mixture may be partitioned from the rermainider of the Candidate Mixture;

b) partitioning the increased affinity VEGF Nucleic Acids from the remnainder of the Candidate Mixture;

c) amplifying the increased affinity VEGF Nucleic Acids to yield a ligand-enriched mixture of Nucleic Acids;

d) covalently linking said identified VEGF Nucleic Acid Ligand with a Lipophilic Compound to form a Complex; and

e) forming a Lipid Construct comprising said Complex.
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