.

Pharmaceutical Business Intelligence

  • Anticipate P&T budget requirements
  • Evaluate market entry opportunities
  • Find generic sources and suppliers
  • Predict branded drug patent expiration

► Plans and Pricing

Upgrade to enjoy subscriber-only features like email alerts and data export. See the Plans and Pricing

DrugPatentWatch Database Preview

Details for Patent: 5,858,660

« Back to Dashboard

Details for Patent: 5,858,660

Title: Parallel selex
Abstract:This invention discloses a method for coevolving products from two or more reactants, along with the nucleic acid that can facilitate the reaction for making the products. The invention further discloses the products and facilitating nucleic acids produced by said method.
Inventor(s): Eaton; Bruce (Boulder, CO), Gold; Larry (Boulder, CO)
Assignee: NeXstar Pharmaceuticlas, Inc. (Boulder, CO)
Filing Date:Mar 20, 1996
Application Number:08/618,700
Claims:1. A method for producing a product having the ability to perform a preselected function on a target comprising:

(a) preparing a nucleic acid test mixture;

(b) coupling each member of said nucleic acid test mixture with a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a nucleic acid-first reactant test mixture;

(c) forming a product library by contacting said nucleic acid-first reactant test mixture with a mixture of free reactants consisting of small organic molecules with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation reaction is facilitated by a nucleic acid coupled to said first reactant;

(d) contacting the product library of step (c) with a target, wherein the product having the ability to perform a preselected function on said target relative to the product library may be partitioned from the remainder of the product library; and

(e) partitioning said product having said ability to perform a preselected function on said target from the remainder of the product library, whereby said product can be identified.

2. A method for coproducing a nucleic acid that facilitates bond formation and a product that performs a preselected function on a target comprising:

(a) preparing a nucleic acid test mixture;

(b) coupling a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to each member of said nucleic acid test mixture to form a nucleic acid-first reactant test mixture;

(c) forming a product library by contacting said nucleic acid-first reactant test mixture with a mixture of free reactants consisting of small organic molecules with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation is facilitated by a nucleic acid which is coupled to said first reactant and wherein each product is coupled to a nucleic acid;

(d) contacting said product library with a target, wherein products that perform a preselected function on said target relative to the product library may be partitioned from the remainder of the product library; and

(e) amplifying the nucleic acid associated with the product that performs a preselected function on said target, to yield a mixture of nucleic acids enriched for nucleic acids that facilitate bond formation between said first reactant and said free reactant, whereby said nucleic acid that facilitates bond formation and said product are produced.

3. The method of claim 2 which further comprises:

(f) coupling the amplified nucleic acids with said first reactant; and

(g) repeating steps (c) through (f) until the product having said ability to perform said preselected function on said target is produced in sufficient quantity for structure determination.

4. A method for producing a product library comprising contacting a mixture of first reactants each coupled to a member of a nucleic acid test mixture of free reactants, each first and free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation reaction is facilitated by the nucleic acid coupled to said first reactant.

5. The method of claim 1 wherein said nucleic acid test mixture comprises nucleic acids having a region of conserved sequences and a region of randomized sequences.

6. The method of claim 1 wherein said nucleic acid coupled to said first reactant is selected from the group consisting of single-stranded RNA, single-stranded DNA and double-stranded DNA.

7. The method of claim 1 wherein said nucleic acid test mixture comprises pyrimidines modified at the 2'- or 5-positions.

8. The method of claim 1 wherein said nucleic acid test mixture comprises purines modified at the 8-position.

9. The method of claim 1 which further comprises a linker group coupled between said first reactant and said nucleic acid.

10. The method of claim 9 wherein said linker group has a size in the range of 10 to 1000.

11. The method of claim 10 wherein said linker group is selected from the group consisting of PEG, polyvinyl alcohol, polyacrylates and polypeptides.

12. The method of claim 1 wherein said first reactant is a dienophile, said free reactant is a diene, and said product is a cyclohexene.

13. The method of claim 2 wherein said nucleic acid test mixture comprises nucleic acids having a region of conserved sequences and a region of randomized sequences.

14. The method of claim 2 wherein said nucleic acid coupled to said first reactant is selected from the group consisting of single-stranded RNA, single-stranded DNA and double-stranded DNA.

15. The method of claim 2 wherein said nucleic acid test mixture comprises pyrimidines at the 2'- or 5- positions.

16. The method of claim 2 wherein said nucleic acid test mixture comprises purines modified at the 8-position.

17. The method of claim 2 which further comprises a linker group coupled between said first reactant and said nucleic acid.

18. The method of claim 17 wherein said linker group has a size in the range of 10 to 1000.

19. The method of claim 18 wherein said linker group is selected from the group consisting of PEG, polyvinyl alcohol, polyacrylates and polypeptides.

20. The method of claim 2 wherein said first reactant is a dienophile, said free reactant is a diene, and said product is a cyclohexene.
« Back to Dashboard

For more information try a trial or see the database preview and plans and pricing

How are People Using DrugPatentWatch?

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.

`abc