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Generated: December 15, 2017

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Title: Nucleic acids encoding and recombinant production of the .beta. subunit of lutenizing hormone
Abstract:Biologically active heterodimeric human fertility hormones composed of two different subunits, each subunit being synthesized in the same cell transformed by at least one cell expression vector having heterologous DNA encoding each subunit with each subunit being controlled by a separate promoter. Preferred human fertility hormones include hCG, hLH and hFSH.
Inventor(s): Reddy; Vermuri B. (Westboro, MA), Hsiung; Nancy (Wellesley, MA), Beck; Anton K. (Pfeffingen, CH), Berstine; Edward George (Boston, MA)
Assignee: Genzyme Corporation (Boston, MA)
Filing Date:Jun 07, 1995
Application Number:08/485,503
Claims:1. Isolated DNA encoding the .beta.-subunit of human luteinizing hormone and having the sequence encoding said human luteinizing hormone which is present in E. coli clone pCL28XhoLHBVP (ATCC 39475).

2. An expression vector containing DNA encoding the .beta.-subunit of human luteinizing hormone and having the sequence encoding said human luteinizing hormone which is present in E. coli clone pCL28XhoLHBVP (ATCC 39475).

3. A cell transformed by an expression vector in accordance with claim 2.

4. A method for the production of a molecule including the .beta.-subunit of human luteinizing hormone comprising culturing a cell in accordance with claim 3 under conditions sufficient to cause expression of said molecule and recovering said molecule from the culture.
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