.

Pharmaceutical Business Intelligence

  • Anticipate P&T budget requirements
  • Evaluate market entry opportunities
  • Find generic sources and suppliers
  • Predict branded drug patent expiration

► Plans and Pricing

Upgrade to enjoy subscriber-only features like email alerts and data export. See the Plans and Pricing

DrugPatentWatch Database Preview

Details for Patent: 5,837,834

« Back to Dashboard

Details for Patent: 5,837,834

Title: High affinity HKGF nucleic acid ligands and inhibitors
Abstract:Methods are described for the identification and preparation of high-affinity nucleic acid ligands to hKGF. Included in the invention are specific RNA ligands to hKGF identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of hKGF with its receptor.
Inventor(s): Pagratis; Nikos (Boulder, CO), Gold; Larry (Boulder, CO)
Assignee: NeXstar Pharmaceuticals, Inc. (Boulder, CO)
Filing Date:Jun 05, 1995
Application Number:08/465,591
Claims:1. A nucleic acid ligand to Human Keratinocyte Growth Factor (hKGF) identified according to the method comprising:

a) preparing a candidate mixture of nucleic acids;

b) contacting the candidate mixture of nucleic acids with hKGF, wherein nucleic acids having an increased affinity to hKGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;

c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and

d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to hKGF, whereby a nucleic acid ligand of hKGF may be identified.

2. A purified and isolated non-naturally occurring RNA ligand to hKGF wherein said ligand is selected from the group consisting of the sequences set forth in Table 3 (SEQ ID NOS. 4-77).

3. A purified and isolated non-naturally occurring nucleic acid ligand to hKGF.

4. The nucleic acid ligand of claim 1 wherein said candidate mixture of nucleic acids is comprised of single stranded nucleic acids.

5. The nucleic acid ligand of claim 4 wherein said single stranded nucleic acids are ribonucleic acids.

6. The nucleic acid ligand of claim 4 wherein said single stranded nucleic acids are deoxyribonucleic acids.

7. The nucleic acid ligand of claim 5 wherein said candidate mixture of nucleic acids comprises 2'-amino (2'-NH.sub.2) modified ribonucleic acids.

8. The nucleic acid ligand of claim 5 wherein said candidate mixture of nucleic acids comprises 2'-F (2'-fluoro) modified ribonucleic acids.

9. The purified and isolated non-naturally occurring nucleic acid ligand of claim 3 wherein said nucleic acid ligand is single-stranded.

10. The purified and isolated non-naturally occurring nucleic acid ligand of claim 9 wherein said nucleic acid ligand is RNA.

11. The purified and isolated non-naturally occurring RNA ligand of claim 10 wherein said ligand is comprised of 2'-amino (2'-NH.sub.2) modified nucleotides.

12. The purified and isolated non-naturally occurring RNA ligand of claim 10 wherein said ligand is comprised of 2'-fluoro (2'-F) modified nucleotides.

13. The purified and isolated non-naturally occurring nucleic acid ligand of claim 9 wherein said nucleic acid ligand is DNA.
« Back to Dashboard

For more information try a trial or see the database preview and plans and pricing

How are People Using DrugPatentWatch?

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.

`abc