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|Title:||Microsomal triglyceride transfer protein|
|Abstract:||Nucleic acid sequences, particularly DNA sequences, coding for all or part of the high molecular weight subunit of microsomal triglyceride transfer protein, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of the high molecular weight subunit of microsomal triglyceride transfer protein, and methods for producing these polypeptide molecules. The invention additionally concerns novel methods for preventing, stabilizing or causing regression of atherosclerosis and therapeutic agents having such activity. The invention concerns further novel methods for lowering serum liquid levels and therapeutic agents having such activity.|
|Inventor(s):||Wetterau, II; John R. (Langhorne, PA), Sharp; Daru Young (Perrineville, NJ), Gregg; Richard E. (Pennington, NJ)|
|Assignee:||E. R. Squibb & Sons, Inc. (Princeton, NJ)|
|Filing Date:||Jun 07, 1995|
|Claims:||1. A method for producing a polypeptide molecule comprising an amino acid sequence of SEQ. ID. NOS. 3, 4, or 3 together with 6, which comprises: |
(a) culturing a host cell under conditions permitting expression of the polypeptide, wherein the host cell comprises an expression vector having a DNA sequence coding for said amino acid sequence; and
(b) recovering therefrom the polypeptide molecule.
2. The method of claim 1, wherein the DNA sequence is selected from SEQ. ID, NOS. 1 and 2.
3. An isolated polypeptide molecule having the amino acid sequence of SEQ. ID. NOS. 3, 4, or 3 together with 6.
4. The polypeptide molecule according to claim 3 having the amino acid sequence of SEQ. ID. NO. 4.
5. A process for detecting an inhibitor of microsomal triglyceride transfer protein comprising:
(a) incubating a sample thought to contain an inhibitor of microsomal triglyceride transfer with detectably labeled lipids in donor particles, acceptor particles and microsomal triglyceride transfer protein; and
(b) measuring the transfer of the detectably labeled lipid from the donor particles to the acceptor particles promoted by the microsomal triglyceride transfer protein; wherein the inhibitor will decrease the rate of transfer of detectably labeled lipid from donor particles to acceptor particles.