|Title:||Method utilizing purified GP IIb-IIIa to detect the presence of platelet aggregation inhibitors|
|Abstract:||An assay for screening snake venom for the presence or absence of platelet aggregation inhibitors (PAIs) based on specific receptor binding is described. Using this assay, the identification and characterization of PAIs in a wide range of snake venom samples was accomplished. The isolated and purified PAI from several of these active snake venoms is described and characterized. In addition, PAIs lacking the Arg-Gly-Asp (RGD) adhesion sequence but containing K*-(G/Sar)-D wherein K* is a modified lysyl residue of the formula R.sup.1.sub.2 N(CH.sub.2).sub.4 CHNHCO-- wherein each R.sup.1 is independently H, alkyl(1-6C) or at most one R.sup.1 is R.sup.2 --C.dbd.NR.sup.3 wherein R.sup.2 is H, alkyl(1-6C) or is NR.sup.4.sub.2 in which each R.sup.4 is independently H or alkyl(1-6C) and R.sup.3 is H, alkyl(1-6C), phenyl or benzyl, or R.sub.2 --C.dbd.NR.sup.3 is a radical selected from the group consisting of: ##STR1## where m is an integer of 2-3, and each R.sup.5 is independently H or alkyl(1-6C); and wherein one or two (CH.sub.2) may be replaced by O or S provided said O or S is not adjacent to another heteroatom are prepared and shown to specifically inhibit the binding of fibrinogen or yon Willebrand Factor to GP IIb-IIIa.|
|Inventor(s):||Scarborough; Robert M. (Belmont, CA), Wolf; David Lawrence (Palo Alto, CA), Charo; Israel F. (Lafayette, CA)|
|Assignee:||COR Therapeutics, Inc. (South San Francisco, CA)|
|Filing Date:||Jun 05, 1995|
|Claims:||1. A method to determine the presence or absence of platelet aggregation inhibitor (PAI) activity in a biological fluid, which method comprises: |
contacting a sample of said fluid with a solid support coated with purified platelet GP IIb-IIIa in the presence of a solution of fibrinogen (Fg) or von Willebrand Factor (vWF) under conditions wherein Fg or vWF binds to said GP IIb-IIIa, and
detecting the decrease or lack of decrease in the binding of Fg or vWF to GP IIb-IIIa in comparison to the binding of Fg or vWF to GP IIb-IIIa in a control which does not contain biological fluid.