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Details for Patent: 5,712,093

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Details for Patent: 5,712,093

Title: RXR homodimer formation
Abstract:The invention provides a method of screening a substance for the ability to affect the formation of a retinoid X receptor homodimer comprising combining the substance and a solution containing retinoid X receptors and determining the presence of homodimer formation. Also provided is a method of screening a substance for an effect on a retinoid X receptor homodimer's ability to bind DNA comprising combining the substance with the homodimer and determining the effect of the compound on the homodimer's ability to bind DNA. A method of inhibiting an activity of a retinoid X receptor heterodimer comprising increasing the formation of a retinoid X receptor homodimer, thereby preventing the retinoid X receptor from forming a heterodimer and preventing the resulting heterodimer activity is also provided. A method of inhibiting an activity of a retinoid X receptor homodimer is also provided. A method of determining an increased probability of a pathology associated with retinoid X receptor homodimer formation and treating such pathology are further provided. Finally a method of screening a response element for binding to a retinoid X receptor homodimer is provided.
Inventor(s): Pfahl; Magnus (Solana Beach, CA), Zhang; Xiao-kun (La Jolla, CA)
Assignee: The Burnham Institute (La Jolla, CA)
Filing Date:Aug 29, 1994
Application Number:08/297,706
Claims:1. A method of screening a substance for the ability to affect the formation of a functional retinoid X receptor homodimer, which affects the activation of transcription by the homodimer comprising combining the substance and a solution containing retinoid X receptors and determining the presence of homodimer formation in solution by either 1) a gel shift assay utilizing a response element to bind the homodimer or 2) coprecipitation, the presence of homodimer formation indicating a substance that can affect the activation of transcription.

2. The method of claim 1, wherein the effect is the induction of homodimer formation.

3. The method of claim 1, wherein the retinoid X receptor is retinoid X receptor .alpha..

4. The method of claim 1, wherein the presence of homodimer formation is detected by said coprecipitation and said coprecipitation is resolved by a denaturing gel electrophoresis assay.

5. The method of claim 1, wherein the presence of homodimer formation is detected by said gel shift assay and said gel shift assay is resolved under non-denaturing conditions.

6. A method of screening a substance for the ability to inhibit the formation of a retinoid X receptor homodimer, which affects the activation of transcription by the homodimer, comprising the steps of:

a. contacting in a first solution, retinoid X receptors and a ligand under conditions that allow retinoid X receptor homodimer formation;

b. determining a first amount of retinoid X receptor homodimer formation in said first solution by a method selected from the group consisting of 1) a gel shift assay utilizing a response element to bind the homodimer and 2) coprecipitation;

c. contacting in a second solution, retinoid X receptors, a ligand and a substance under said conditions of step a);

d. determining a second amount of retinoid X receptor homodimer formation in said second solution by the method selected in step b); and

e. thereafter comparing said first amount of retinoid X receptor homodimer formation and said second amount of retinoid X receptor homodimer formation, wherein a determination that said first amount is greater than said second amount indicates said substance inhibits the formation of a retinoid X receptor homodimer, the inhibition of homodimer formation indicating a substance that can affect the activation of transcription.

7. A method of screening a response element for binding with a retinoid X receptor homodimer, comprising combining in solution the response element with the retinoid X receptor homodimer complexed with a ligand selected from the group consisting of 9-cis-RA and all-trans-RA, and detecting the presence of binding by a gel shift assay.

8. The method of claim 7, wherein said gel shift assay is resolved under non-denaturing conditions.
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