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|Title:||High affinity HIV nucleocapsid nucleic acid ligands|
|Abstract:||Methods are described for the identification and preparation of high-affinity nucleic acid ligands to HIV-1 nucleocapsid. Included in the invention are specific RNA ligands to HIV-1 nucleocapsid identified by the SELEX method and RNA ligands that inhibit the function of HIV-1 nucleocapsid.|
|Inventor(s):||Allen; Patrick (Boulder, CO), Gold; Larry (Boulder, CO)|
|Assignee:||NeXstar Pharmaceuticals, Inc. (Boulder, CO)|
|Filing Date:||Jun 07, 1995|
|Claims:||1. A purified and isolated non-naturally occurring nucleic acid ligand to HIV-1 nucleocapsid. |
2. The purified and isolated non-naturally occurring nucleic acid ligand of claim 1 wherein said nucleic acid ligand is single-stranded.
3. The purified and isolated non-naturally occurring nucleic acid ligand of claim 2 wherein said nucleic acid ligand is RNA.
4. The purified and isolated non-naturally occurring nucleic acid ligand of claim 2 wherein said nucleic acid ligand is DNA.
5. A nucleic acid ligand to HIV-1 nucleocapsid identified according to the method comprising:
a) preparing a candidate mixture of nucleic acids;
b) contacting the candidate mixture of nucleic acids with HIV-1 nucleocapsid, wherein nucleic acids having an increased affinity to HIV-1 nucleocapsid relative to the candidate mixture may by partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to HIV-1 nucleocapsid, whereby "a nucleic acid ligand" of HIV-1 nucleocapsid may be identified.
6. The nucleic acid ligand of claim 5 wherein the candidate mixture contacted includes non-amplifiable random pool nucleic acids.
7. The purified and isolated non-naturally occurring RNA ligand to HIV-1 nucleocapsid of claim 3 wherein said ligand is selected from the group consisting of the sequences set forth in Table 2.
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