.

Pharmaceutical Business Intelligence

  • Anticipate P&T budget requirements
  • Evaluate market entry opportunities
  • Find generic sources and suppliers
  • Predict branded drug patent expiration

► Plans and Pricing

Upgrade to enjoy subscriber-only features like email alerts and data export. See the Plans and Pricing

DrugPatentWatch Database Preview

Details for Patent: 5,635,604

« Back to Dashboard

Details for Patent: 5,635,604

Title: Amino-terminally extended human growth hormone (HGH)
Abstract:A desired protein having the formula: wherein (a) A is Lys or Arg, and B and C are arbitrary amino acids, or (b) A is an arbitrary amino acid different from Pro, Lys, and Arg, and B and/or C is Pro is produced from a biosynthetically formed amino-terminally extended protein having the formula: wherein A, B, C are defined above, and X is an amino acid sequence with an even number of amino acids, of which the first amino acid, seen from the N-terminal end, is different from Lys and Arg, all other am/no acids are different from Pro, Lys and Arg, and all even amino acids are different from Pro, by reaction with the enzyme dipeptidyl aminopeptidase (DAP I). The desired protein is obtained in a pure state. Specifically, P is human growth hormone (hGH). Thus, compositions of hGH may be produced without the presence of Met-hGH.
Inventor(s): Dalb.o slashed.ge; Henrik (Virum, DK), Pedersen; John (Kokkedal, DK), Christensen; Thorkild (Aller.o slashed.d, DK), Ringsted; J.o slashed.rli W. (Br.o slashed.ndby, DK), Jessen; Torben E. (Holbaek, DK)
Assignee: Novo Nordisk A/S (DK)
Filing Date:May 25, 1995
Application Number:08/450,094
Claims:1. A substantially pure amino-terminal extended human growth hormone of the formula:

X-human growth hormone wherein X is a charged amino acid sequence having at least 2 amino acids and wherein the N-terminal amino acid of X is other than LyS and Arg, and wherein all other uneven numbered amino acids are other than Pro, Lys and Arg, and wherein all even numbered amino acids are other than Pro, and wherein at least one amino acid in amino acid sequence X is a negatively charged amino acid.

2. An amino-terminal extended human growth hormone according to claim 1 wherein X comprises an even number of amino acids.

3. An amino-terminal extended human growth hormone according to claim 1 wherein the C-terminal amino acid of X is a charged amino acid.

4. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X comprises 4 amino acids.

5. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X comprises 2 amino acids.

6. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X is Met-Glu-Ala-Glu.

7. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X is Met-Phe-Glu-Glu.

8. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X is Met-Thr-Glu-Glu.

9. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X is Met-Glu-Glu-Glu.

10. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence X is Ala-Ala-Glu-Glu.

11. An amino-terminal extended human growth hormone according to claim 1 wherein the N-terminal amino acid of X is Met.

12. An amino-terminal extended human growth hormone according to claim 1 wherein the C-terminal amino acid of X is Glu or Asp.

13. An amino-terminal extended human growth hormone according to claim 1 wherein the amino acid sequence x is Ala-Glu.
« Back to Dashboard

For more information try a trial or see the database preview and plans and pricing

How are People Using DrugPatentWatch?

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.

`abc