|Title:|| Method for identification of high affinity DNA ligands of HIV-1 reverse transcriptase|
|Abstract:||Methods are described for the identification and preparation of DNA ligands to the HIV-1 reverse transcriptase protein. The invention encompasses specific ssDNA ligands to HIV-1 reverse transcriptase identified by the SELEX method including ssDNA ligands that inhibit the activity of HIV-1 reverse transcriptase.|
|Inventor(s):|| Schneider; Daniel J. (Boulder, CO), Gold; Larry (Boulder, CO), Feigon; Juli (Los Angeles, CA) |
|Assignee:|| University Research Corporation (Boulder, CO) |
|Filing Date:||May 06, 1994|
|Claims:||1. A method of identifying nucleic acid ligands to HIV-1 reverse transcriptase, comprising: |
a) preparing a candidate mixture of deoxyribonucleic acids (DNA);
b) contacting the candidate mixture of DNA with HIV-1 reverse transcriptase, wherein nucleic acids having an increased affinity to HIV-1 reverse transcriptase relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acids to yield a ligand-enriched mixture of nucleic acids, whereby nucleic acid ligands to HIV-1 reverse transcriptase may be identified.
2. The method of claim 1 further comprising:
(e) repeating steps b), c) and d).
3. The method of claim 1 wherein said candidate mixture of DNA is comprised of single stranded DNA.