|Title:|| Expression of biologically active PDGE analogs in eucaryotic cells|
|Abstract:||Methods for expressing a variety of biologically active PDGF analogs in eucaryotic cells are disclosed. The methods generally comprise introducing into a eucaryotic host cell a DNA construct capable of directing the expression and secretion of biologically active PDGF analogs in eucaryotic cells. The DNA construct contains a transcriptional promoter followed downstream by a suitable DNA sequence. The DNA sequence may encode a protein substantially homologous to the A-chain or the B-chain of PDGF, or a portion thereof, or an A-B heterodimer. In addition, a portion of the DNA sequence may encode at least a portion of the A-chain, while another portion encodes at least a portion of the B-chain of PDGF. Eucaryotic cells transformed with these DNA constructs are also disclosed. Methods of promoting the growth of mammalian cells, comprising incubating the cells with a biologically active PDGF analog expressed by a eucaryotic host cell transformed with such a DNA construct, are also disclosed.|
|Inventor(s):|| Murray; Mark J. (Seattle, WA), Kelly; James D. (Seattle, WA) |
|Assignee:|| ZymoGenetics, Inc. (Seattle, WA) |
|Filing Date:||Jan 02, 1992|
|Claims:||1. A mitogenic protein produced by a method comprising: |
(a) introducing into a eucaryotic host cell a DNA construct capable of directing the expression and secretion of biologically active PDGF analogs in eucaryotic cells, said DNA construct containing a transcriptional promoter followed downstream by a DNA sequence that encodes the B-chain of a primate PDGF; and
(b) isolating from the host cell a mitogenic protein, which is a PDGF BB Romodimer, expressed from said DNA construct.