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Details for Patent: 4,840,896

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Details for Patent: 4,840,896

Title: Heteropolymeric protein
Abstract:Biologically active heteropolymeric protein composed of a plurality of subunits, each subunit being synthesized by a cell having an expression vector heterologous DNA encoding the subunit.
Inventor(s): Reddy; Vermuri B. (Framingham, MA), Hsiung; Nancy (Wellesley, MA), Beck; Anton K. (Chestnut Hill, MA), Bernstine; Edward G. (Boston, MA)
Assignee: Integrated Genetics, Inc. (Framingham, MA)
Filing Date:Nov 02, 1983
Application Number:06/548,228
Claims:1. A mammalian cell comprising a first expression vector, said cell being capable of producing a biologically active heterodimeric human fertility hormone comprised of an alpha subunit and a beta subunit, each said subunit being encoded in nature by a distinct mRNA, said hormone being selected from the group consisting of hCG, and LH, the alpha subunit of said hormone being encoded by said first expression vector and the beta subunit being encoded by said first expression vector or a second expression vector.

2. The cell of claim 1, further comprising a second expression vector encoding the beta subunit of said hormone.

3. The cell of claim 2, said second expression vector being autonomously replicating.

4. The cell of claim 1, said first vector being a plasmid.

5. The cell of claim 4, said cell being a monkey cell.

6. The cell of claim 1, the alpha and beta subunits of sid heterodimeric hormone being encoded by said first expression vector.

7. The cell of claim 6, transcription of said alpha and beta subunits of said heterodimeric hormone being under the control of the SV40 late promoter.

8. The cell of claim 6, transcription of the alpha subunit of said heterodimeric hormone being under the control of the SV40 early promoter, and transcription of the beta subunit of said heterodimeric hormone being under the control of the mouse metallothionein promoter.

9. The cell of claim 8, said first vector comprising at least the 69% transforming region of the bovine papilloma virus genome.

10. The cell of claim 1, transcription of the alpha and beta subunits of said heterodimeric hormone being under the control of the SV40 late promoter.

11. The cell of claim 1, transcription of the alpha and beta subunits of said heterodimeric hormone being under the control of the mouse metallothionein promoter.

12. The cell of claim 1, said cell being a mouse cell.

13. The cell of claim 1, said first expression vector being autonomously replicating.

14. A method for producing the biologically active human fertility hormone LH comprising culturing host mammalian cells comprising a first expression vector encoding the beta subunit of said LH and a second expression vector encoding the alpha subunit of said LH.

15. The method of claim 14, wherein each said expression vector is autonomously replicating.

16. A method for producing biologically active hCG comprising culturing host mammalian cells comprising an expression vector encoding the alpha and beta subunits of said hCG.

17. A method for producing biologically active hCG comprising culturing host mammalian cells comprising a first expression vector encoding the alpha subunit of said hCG and a second expression vector encoding the beta subunit of said hCG.

18. A method for producing the biologically active human fertility hormone LH comprising culturing host mammalian cells comprising an expression vector encoding both the beta and alpha subunits of said LH.
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