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|Title:||Preparation of ammonium salt of hypocholesteremic fermentation product|
|Abstract:||Substances isolated after cultivation of a microorganism belonging to the genus Aspergillus in a culture medium comprise compounds which have structures: ##STR1## Together with salts and esters of the carboxylic acid, these compounds form a class of highly active hypocholesteremic and hypolipemic medicaments.|
|Inventor(s):||Albers-Schonberg; George (Princeton, NJ)|
|Assignee:||Merck & Co., Inc. (Rahway, NJ)|
|Filing Date:||Aug 11, 1980|
|Claims:||1. A process for the isolation of the compound ##STR6## as its ammonium salt from a fermentation broth obtained from the cultivation of microorganisms identified as Aspergillus terreus with Accession numbers ATCC 20541 and ATCC 20542 which comprises the steps of: |
(a) acidifying whole broth;
(b) extracting with a water-immiscible, inert, organic solvent;
(c) extracting the organic solvent extract with aqueous alkali;
(d) acidifying the aqueous extract;
(e) extracting the aqueous extract with a water-immiscible, inert, organic solvent;
(f) drying the organic solvent extract; and
(g) treating the organic solvent extract with gaseous ammonia.
2. The process of claim 1 wherein acidification is with phosphoric acid to about pH 5 and the immiscible organic solvent is ethyl acetate or mixtures of n-hexane-ethyl acetate.