.

Pharmaceutical Business Intelligence

  • Anticipate P&T budget requirements
  • Evaluate market entry opportunities
  • Find generic sources and suppliers
  • Predict branded drug patent expiration

► Plans and Pricing

Upgrade to enjoy subscriber-only features like email alerts and data export. See the Plans and Pricing

DrugPatentWatch Database Preview

Claims for Patent: 5,681,809

« Back to Dashboard

Claims for Patent: 5,681,809

Title: Growth hormone antagonists
Abstract:The present invention relates to antagonists of vertebrate growth hormones obtained by mutation of the third alpha helix of such proteins (especially bovine or human GHs). These mutants have growth inhibitory or other GH-antagonizing effects. These novel hormones may be administered exogenously to animals, or transgenic animals may be made that express the antagonist. Animals have been made which exhibited a reduced growth phenotype.
Inventor(s): Kopchick; John J. (Athens, OH), Chen; Wen Y. (Athens, OH)
Assignee: Ohio University (Athens, OH)
Application Number:08/313,505
Patent Claims: 1. A vertebrate growth hormone in which there are at least two substitutions at amino acid positions corresponding to the amino acid positions of bovine growth hormone, said substitutions being at Gly119, with an amino acid other than alanine, and at least one amino acid position selected from the group consisting of Ala122, Leu123, Ile120, Leu116, Asp115 and Glu118, said vertebrate growth hormone having growth hormone reception antagonist activity.

2. A polypeptide which comprises an amino acid sequence which

(A) is at least 50% identical with the sequence of a first reference vertebrate growth hormone, and

(B) differs therefrom solely in that

(I) the amino acid position corresponding to amino acid Gly119 of bovine growth hormone is substituted with an amino acid other than alanine, and either

(II) at one or more of the remaining amino acid positions it is characterized by

(a) a substitution of a conservative replacement amino acid for the corresponding first reference vertebrate growth hormone residue, or

(b) a substitution of a non-conservative replacement amino acid for the corresponding first reference vertebrate growth hormone residue where

(i) a second reference vertebrate growth hormone exists for which the corresponding amino acid is a non-conservative substitution for the corresponding first reference vertebrate growth hormone residue, and/or

(ii) the binding affinity for the first reference vertebrate growth hormone's receptor of a single substitution mutant of the first reference vertebrate growth hormone, wherein said corresponding residue, which is not alanine, is replaced by alanine, is at least 10% of the binding affinity of the wild-type first reference vertebrate growth hormone, or

(III) it differs from the sequence of said first reference hormone by (a) one or more deletions of residues which are not part of the alpha helixes of said reference vertebrate growth hormone corresponding to helices 1(7-34), 2(75-87), 3(106-127) and 4(152-183) of porcine growth hormone, each deleted residue furthermore not being a conserved residue in the vertebrate GH family, or (b) one or more deletions of residues found in said first reference vertebrate growth hormone but deleted in a second reference vertebrate growth hormone, or both (II) and (III);

said polypeptide having growth hormone receptor antagonist activity.

3. The polypeptide of claim 2 wherein, for all non-conservative substitutions, both of conditions (II)(b)(i) and (II)(b)(ii) apply.

4. The polypeptide of claim 2 wherein all substitutions are conservative substitutions as defined in II(a).

5. The polypeptide of claim 2, said amino acid sequence having at least about a 66% identity with the sequence of said first reference vertebrate growth hormone.

6. The polypeptide of claim 2, said amino acid sequence having at least about a 80% identity with the sequence of said first reference vertebrate growth hormone.

7. The polypeptide of claim 2, wherein substitutions of residues in the third alpha helix are limited to residues corresponding to bGH residues Gly119, Ala122, Leu123, Ile120, Leu116, Asp115 and Glu118.

8. The polypeptide of claim 2, wherein the third alpha helix is at least 50% identical to the third alpha helix of said first reference vertebrate growth hormone.

9. The polypeptide of claim 2, wherein the third alpha helix is at least 80% identical to the third alpha helix of said first reference vertebrate growth hormone.

10. The polypeptide of claim 2, wherein the non-conservative substitutions are all of residues other than those belonging to conserved domains GD1 (hGH 9-31), GD2 (hGH 53-68), GD3 (hGH 75-93), GD4 (hGH 114-130) and GD5 (hGH 162-189).

11. The polypeptide of claim 2, wherein the non-conservative substitutions are all of surface residues.

12. The polypeptide of claim 2, wherein each non-conservative substitution is with a replacement amino acid found at the corresponding position in a vertebrate growth hormone, prolactin, placental lactogen, or other hormone homologous to human growth hormone, or with an amino acid which is a member of the same exchange group as an amino acid found at the corresponding position in a hormone homologous to human growth hormone.

13. The polypeptide of claim 2, wherein each non-conservative substitution is with a replacement amino acid found at the corresponding position in a second vertebrate growth hormone, or with an amino acid which is a member of the same exchange group as an amino acid found at that the corresponding position in a second vertebrate growth hormone.

14. The polypeptide of claim 2, wherein each non-conservative substitution is with a replacement amino acid found at the corresponding position in a vertebrate growth hormone, prolactin, placental lactogen, or other hormone homologous to human growth hormone.

15. The polypeptide of claim 2, wherein each non-conservative substitution is with a replacement amino acid found at the corresponding position in a second vertebrate growth hormone.

16. The polypeptide of claim 2 in which the first reference vertebrate growth hormone is a mammalian growth hormone.

17. The polypeptide of claim 2 in which the first reference vertebrate growth hormone is human or bovine growth hormone.

18. The polypeptide of claim 2 in which the substitution (I) is with an amino acid at least as large as proline.

19. The polypeptide of claim 2 in which the substitution (I) is with an amino acid selected from the group consisting of Arg, Trp, Pro, Lys and Leu.

20. The polypeptide of claim 2 which has an ED50 which is less than about 10 times the ED50 of the first reference vertebrate growth hormone in an assay of the ability of the polypeptide to displace radiolabeled first reference vertebrate growth hormone from a liver membrane vertebrate growth hormone receptor.

21. The polypeptide of claim 2 wherein the amino acid position corresponding to amino acid Gly 119 of bovine growth hormone is substituted with an amino acid other than proline.

22. The polypeptide of claim 2 wherein the mutations include at least one substitution according to (II) above which is of a residue which is part of an alpha helix of said reference vertebrate growth hormone and which substitute amino acid has a greater alpha helical propensity than did the corresponding residue of said reference vertebrate growth hormone.

23. The polypeptide of claim 22 wherein said helix corresponds to the third alpha helix of bovine growth hormone.

24. A polypeptide which comprises an amino acid sequence which

(A) is at least 50% identical with the sequence of a first reference vertebrate growth hormone, and

(B) differs therefrom solely in that

(I) the amino acid position corresponding to amino acid Gly119 of bovine growth hormone is substituted with an amino acid other than alanine, and either

(II) at one or more of the remaining amino acid positions it is characterized by

(a) a substitution of a conservative replacement amino acid for the corresponding first reference vertebrate growth hormone residue, or

(b) a substitution of a non-conservative replacement amino acid for the corresponding first reference vertebrate growth hormone residue where

(i) a second reference vertebrate growth hormone exists for which the corresponding amino acid is a non-conservatiVe substitution for the corresponding first reference vertebrate growth hormone residue, and/or

(ii) the binding affinity for the first reference vertebrate growth hormone's receptor of a single substitution mutant of the first reference vertebrate growth hormone, wherein said corresponding residue, which is not alanine, is replaced by alanine, is at least 10% of the binding affinity of the wild-type first reference vertebrate growth hormone, or

(III) it differs from the sequence of said first reference hormone by (a) one or more deletions of residues which are not part of the alpha helixes of said reference vertebrate growth hormone corresponding to helices 1(7-34), 2(75-87), 3(106-127) and 4(152-183) of porcine growth hormone, each deleted residue furthermore not being a conserved residue in the vertebrate GH family, or (b) one or more deletions of residues found in said first reference vertebrate growth hormone but deleted in a second reference vertebrate-growth hormone, or both (II) and (III);

said polypeptide having a statistically significant inhibitory effect on the growth of transgenic mice engineered to produce said polypeptide, as compared to the growth of equivalent nontransgenic mice.

25. The polypeptide of claim 24 wherein the ratio of the growth rate of two month old transgenic mice expressing the polypeptide, to that of their nontransgenic litter mates, is not more than 0.96:1.

26. A polypeptide which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence of a reference vertebrate growth hormone, and wherein the amino acid position corresponding to amino acid Gly 119 of bovine growth hormone is substituted with an amino acid other than alanine, said polypeptide having growth hormone antagonist activity.

27. The polypeptide of claim 26 wherein said amino acid sequence is at least 66% identical to the amino acid sequence of a reference vertebrate growth hormone.

28. The polypeptide of claim 26 wherein said amino acid sequence is at least 80% identical to the amino acid sequence of a reference vertebrate growth hormone.

29. The polypeptide of claim 26 wherein said amino acid sequence is at least about 90% identical to the amino acid sequence of a reference vertebrate growth hormone.

30. The polypeptide of claim 26 wherein said reference amino acid sequence is human or bovine growth hormone.

31. A method of preventing or treating a condition of a human or animal subject characterized by excessive or undesirable growth hormone activity which comprises administering to the subject a growth hormone activity-antagonizing amount of the polypeptide of claim 2.

32. The method of claim 31 wherein the condition is characterized by an excessive growth rate, and the antagonist has a growth-inhibitory effect.

33. The method of claim 31 wherein the condition is diabetes.

34. The method of claim 33 in which the proliferation of microvascular cells otherwise stimulated by endogenous growth hormone is inhibited.

35. The method of claim 34 in which the cells are retinal endothelial cells.

36. The method of claim 31 in which the antagonist inhibits the development of glomerulosclerosis.

37. A method of reducing serum cholesterol levels in a mammal comprising administering to the mammal a polypeptide according to claim 2 in an amount effective to reduce serum levels of cholesterol.

38. The method of claim 31 wherein the condition is a tumor whose growth is stimulated by endogenous growth hormone.
« Back to Dashboard

For more information try a trial or see the database preview and plans and pricing

How are People Using DrugPatentWatch?

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.

`abc