Patent: 7,964,402
✉ Email this page to a colleague
Summary for Patent: 7,964,402
| Title: | Methods for culture and production of single cell populations of human embryonic stem cells |
| Abstract: | We used Accutase.TM., a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency. |
| Inventor(s): | Terskikh; Alexey (San Diego, CA), Bajpai; Ruchi (Redwood City, CA) |
| Assignee: | Sanford-Burnham Medical Research Institute (La Jolla, CA) |
| Application Number: | 11/807,223 |
| Patent Claims: | see list of patent claims |
Details for Patent 7,964,402
| Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
|---|---|---|---|---|---|---|---|
| Smith & Nephew, Inc. | SANTYL | collagenase | Ointment | 101995 | 06/04/1965 | ⤷ Try a Trial | 2026-05-25 |
| >Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
