Patent: 9,964,545
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Summary for Patent: 9,964,545
Title: | Methods for quantifying polypeptides using mass spectrometry |
Abstract: | A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole. |
Inventor(s): | Chambers; Erin E. (North Brookfield, MA), Stapels; Martha (Bellingham, MA), Mather; Joanne (Millis, MA) |
Assignee: | WATERS TECHNOLOGIES CORPORATION (Milford, MA) |
Application Number: | 15/447,838 |
Patent Claims: | see list of patent claims |
Details for Patent 9,964,545
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Novo Nordisk Inc. | NOVOLOG | insulin aspart | Injection | 020986 | 06/07/2000 | ⤷ Try a Trial | 2032-04-18 |
Novo Nordisk Inc. | NOVOLOG | insulin aspart | Injection | 020986 | 01/19/2001 | ⤷ Try a Trial | 2032-04-18 |
Novo Nordisk Inc. | NOVOLOG | insulin aspart | Injection | 020986 | 04/23/2004 | ⤷ Try a Trial | 2032-04-18 |
Novo Nordisk Inc. | NOVOLOG | insulin aspart | Injection | 020986 | 10/31/2013 | ⤷ Try a Trial | 2032-04-18 |
Sanofi-aventis U.s. Llc | LANTUS | insulin glargine | Injection | 021081 | 04/20/2000 | ⤷ Try a Trial | 2032-04-18 |
Sanofi-aventis U.s. Llc | LANTUS | insulin glargine | Injection | 021081 | 04/25/2007 | ⤷ Try a Trial | 2032-04-18 |
Novo Nordisk Inc. | LEVEMIR | insulin detemir | Injection | 021536 | 06/16/2005 | ⤷ Try a Trial | 2032-04-18 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |