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Generated: August 19, 2019

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Patent: 9,856,454

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Summary for Patent: 9,856,454
Title:Rapid mincing of adipose tissues to isolate live cells in vitro
Abstract: A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability.
Inventor(s): Sun; Li-Yi (Hualien County, TW), Li; Dian-Kun (Hualien County, TW), Pang; Cheng-Yoong (Hualien County, TW), Chang; Yao-Jen (Hualien County, TW)
Assignee: Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation (Hualien, TW)
Application Number:14/191,675
Patent Claims:see list of patent claims

Details for Patent 9,856,454

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Advance Biofactures SANTYL collagenase VIAL 101995 001 1965-06-04   Try a Free Trial Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation (Hualien, TW) 2038-01-31 RX search
Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source

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