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Last Updated: October 26, 2020

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Patent: 9,222,081

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Summary for Patent: 9,222,081
Title:Method of producing and purifying an active soluble sialyltransferase
Abstract: The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a N-Acetylgalactosamine (Gal NAc)-.alpha.-2,6-sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase, especially ST6GalNAcI, can be employed for the glycosylation of therapeutic proteins such as G-CSF.
Inventor(s): Angermann; Axel (Dreieich, DE), Scheckermann; Christian (Ehrenkirchen, DE), Schmidt; Karsten (Karlsruhe, DE)
Assignee: BIOGENERIX GMBH (Ulm, DE)
Application Number:13/813,804
Patent Claims:see list of patent claims

Details for Patent 9,222,081

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Start Trial BIOGENERIX GMBH (Ulm, DE) 2030-08-02 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Start Trial BIOGENERIX GMBH (Ulm, DE) 2030-08-02 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Start Trial BIOGENERIX GMBH (Ulm, DE) 2030-08-02 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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