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Last Updated: September 24, 2020

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Patent: 9,012,183

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Summary for Patent: 9,012,183
Title:Use of template switching for DNA synthesis
Abstract: A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3\' end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.
Inventor(s): Lambowitz; Alan M. (Austin, TX), Mohr; Sabine (Austin, TX), White; Travis B. (Austin, TX), Kuersten; Scott (Fitchburg, WI)
Assignee: Board of Regents, The University of Texas System (Austin, TX)
Application Number:14/000,513
Patent Claims:see list of patent claims

Details for Patent 9,012,183

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Start Trial Board of Regents, The University of Texas System (Austin, TX) 2031-02-23 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Start Trial Board of Regents, The University of Texas System (Austin, TX) 2031-02-23 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Start Trial Board of Regents, The University of Texas System (Austin, TX) 2031-02-23 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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