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Generated: September 24, 2019

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Patent: 8,980,580

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Summary for Patent: 8,980,580
Title:Heterologous expression of fungal polyketide synthetic gene in yeast and a method of preparing a compound produced by a protean encoded by the polyketide synthetic gene by the heterologous expression
Abstract: The present invention relates to a method of removing an intron contained in a gene from a eukaryotic gene, and linking only the exon sequences to prepare an expression vector comprising the linked sequences. Specifically, the invention relates to a method of preparing an expression vector containing linked exon sequences comprising amplifying exon sequences by PCR as one or more fragments from a giant fungal gene containing an intron, and linking the fragments together with a restriction enzyme-treated vector using the gap repair cloning method; a method of preparing an expression vector containing a full-length cDNA sequence by synthesizing and linking cDNA fragments from a fungal giant gene; a transformant having introduced therein an expression vector prepared by the method; a protein produced by the transformant; and a method of preparing a compound produced by the protein using the expression vector.
Inventor(s): Watanabe; Kenji (Shizuoka, JP), Moriya; Hisao (Okayama, JP)
Assignee: Shizuoka Prefecture Public University Corporation (Shizukoa, JP) National University Corporation Okayama University (Okayama, JP)
Application Number:13/816,826
Patent Claims:see list of patent claims

Details for Patent 8,980,580

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Try a Free Trial Shizuoka Prefecture Public University Corporation (Shizukoa, JP) National University Corporation Okayama University (Okayama, JP) 2030-08-13 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Try a Free Trial Shizuoka Prefecture Public University Corporation (Shizukoa, JP) National University Corporation Okayama University (Okayama, JP) 2030-08-13 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Try a Free Trial Shizuoka Prefecture Public University Corporation (Shizukoa, JP) National University Corporation Okayama University (Okayama, JP) 2030-08-13 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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