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Last Updated: September 27, 2020

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Patent: 8,546,136

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Summary for Patent: 8,546,136
Title:Compositions and methods for the assembly of polynucleotides
Abstract: The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with promer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides.
Inventor(s): Serber; Zach (Emeryville, CA), Lowe; Raymond (Emeryville, CA), Ubersax; Jeffrey A. (Emeryville, CA), Chandran; Sunil S. (Emeryville, CA)
Assignee: Amyris, Inc. (Emeryville, CA)
Application Number:13/430,322
Patent Claims:see list of patent claims

Details for Patent 8,546,136

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Start Trial Amyris, Inc. (Emeryville, CA) 2028-11-19 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Start Trial Amyris, Inc. (Emeryville, CA) 2028-11-19 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Start Trial Amyris, Inc. (Emeryville, CA) 2028-11-19 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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