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Last Updated: November 27, 2021

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Patent: 7,964,402

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Summary for Patent: 7,964,402
Title:Methods for culture and production of single cell populations of human embryonic stem cells
Abstract: We used Accutase.TM., a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.
Inventor(s): Terskikh; Alexey (San Diego, CA), Bajpai; Ruchi (Redwood City, CA)
Assignee: Sanford-Burnham Medical Research Institute (La Jolla, CA)
Application Number:11/807,223
Patent Claims:see list of patent claims

Details for Patent 7,964,402

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Smith & Nephew, Inc. SANTYL collagenase Ointment 101995 1965-06-04 ⤷  Try it Free 2026-05-25
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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