Method for producing a compound of interest in a filamentous fungal cell
The invention relates to a nucleotide sequence comprising; a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, the synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally the nucleotide sequence comprises control sequences such as; one translational termination sequence orientated in 5\' towards 3\' direction selected from the following list of sequences; TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5\' towards 3\' direction selected from the following list of sequences; gctnccyyc (SEQ ID NO:20), using ambiguity codes for nucleotides; v (A/C/G); n (A/C/G/T), preferably 5\'-GCT TCC TTC-3\' (SEQ ID NO:21). The invention further relates to a consensus translational initiator sequence; 5\'-mwChkyCAmv-3\' (SEQ ID NO:16), preferably the translational initiator sequence is selected from the list consisting of; 5\'-mwChkyCAAA-3\' (SEQ ID NO:17), 5\'-mwChkyCACA-3\' (SEQ ID NO:18), and 5\'-mwChkyCAAG-3\' (SEQ ID NO:19).
Roubos; Johannes Andries (Pijnacker, NL), Donkers; Serge Petrus (Hellevoetsluis, NL), Stam; Hein (Huizen, NL), Peij; Noel Nicolaas Maria Elisabeth Van (Delft, NL)
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