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Patent: 7,601,520
Summary for Patent: 7,601,520
| Title: | Method for rapid identification of porcine insulin-like growth factor 2 intron 7 point mutation |
| Abstract: | A method for rapidly identifying a point mutation in porcine insulin-like-growth factor 2 intron 7 uses published primers to amplify the target DNA fragments by polymerase chain reaction. The DNA fragments are cloned and sequenced for confirmation and method validation. The key positions of the sequence are modified to generate three primers for amplifying different DNA fragments with different genotypes by PCR to avoid additional restriction enzyme digestion. A long primer is used to specifically amplify the lean muscle mass-enhancing allele, a short primer is used to specifically amplify the lean muscle mass-suppressing allele, and the third primer is shared and anneals to the complementary strand. After PCR and electrophoresis, samples with only the 92 bp band are identified as the CC genotype, samples with only the 72 bp band are identified as the GG genotype, and samples with both 92 bp and 72 bp bands are identified as heterozygotes. |
| Inventor(s): | Chang; Hsiu-Luan (Neipu Township, Pingtung County, TW), Wu; Ming-Che (Neipu Township, Pingtung County, TW), Chu; Chia Te (Neipu Township, Pingtung County, TW) |
| Assignee: | |
| Application Number: | 11/598,659 |
| Patent Claims: | see list of patent claims |
Details for Patent 7,601,520
| Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
|---|---|---|---|---|---|---|---|
| Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | 1986-06-04 | ⤷ Free Forever Trial | 2039-02-26 |
| Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | ⤷ Free Forever Trial | 2039-02-26 | |
| >Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
