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Last Updated: September 18, 2020

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Patent: 6,635,458

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Summary for Patent: 6,635,458
Title: Enzymatic process for the preparation of cephalosporanic 7$B(G)-(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
Abstract:Enzymatic process for the preparation of cephalosporanic 7.beta.-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5\' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.
Inventor(s): Garcia Lopez; Jose Luis (Madrid, ES), Cortes Rubio; Estrella (Madrid, ES), Alonso Palacios; Jorge (Madrid, ES), Mellado Duran; Encarnacion (Leon, ES), Diez Garcia; Bruno (Leon, ES), Guisan Seijas; Jose Manuel (Madrid, ES), Salto Maldonado; Franciso (Madrid, ES), Fuente; Barredo (Leon, ES)
Assignee: Antibioticos, S.A.U. (Leon, ES)
Application Number:09/308,683
Patent Claims:see list of patent claims

Details for Patent 6,635,458

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Start Trial Antibioticos, S.A.U. (Leon, ES) 2017-09-25 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Start Trial Antibioticos, S.A.U. (Leon, ES) 2017-09-25 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Start Trial Antibioticos, S.A.U. (Leon, ES) 2017-09-25 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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