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Last Updated: October 1, 2020

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Patent: 6,541,207

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Summary for Patent: 6,541,207
Title: Methods for generating recombined polynucleotides
Abstract:A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, whereby A. extended primers are synthesized by a) denaturing the DNA templates b) annealing primers to the templates, c) extending the said primers by use of a polymerase, d) stop the synthesis, and e) separate the extended primers from the templates, B. a template shift is induced by a) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, or b) repeating steps A.b) to A.e), C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof. Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.
Inventor(s): Vind; Jesper (V.ae butted.rl.o slashed.se, DK), Borchert; Torben Vedel (.O slashed.sterbro, DK)
Assignee: Novozymes A/S (Bagsvaerd, DK)
Application Number:09/687,301
Patent Claims:see list of patent claims

Details for Patent 6,541,207

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Nps Pharms Inc NATPARA parathyroid hormone INJECTABLE;INJECTION 125511 004 2015-01-23   Start Trial Novozymes A/S (Bagsvaerd, DK) 2017-03-18 RX Orphan search
Nps Pharms Inc NATPARA parathyroid hormone INJECTABLE;INJECTION 125511 003 2015-01-23   Start Trial Novozymes A/S (Bagsvaerd, DK) 2017-03-18 RX Orphan search
Nps Pharms Inc NATPARA parathyroid hormone INJECTABLE;INJECTION 125511 002 2015-01-23   Start Trial Novozymes A/S (Bagsvaerd, DK) 2017-03-18 RX Orphan search
Nps Pharms Inc NATPARA parathyroid hormone INJECTABLE;INJECTION 125511 001 2015-01-23   Start Trial Novozymes A/S (Bagsvaerd, DK) 2017-03-18 RX Orphan search
Fresenius Kabi Usa CHORIONIC GONADOTROPIN gonadotropin, chorionic INJECTABLE;INJECTION 017067 004 1973-03-05   Start Trial Novozymes A/S (Bagsvaerd, DK) 2017-03-18 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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