Methods comprising apoptosis inhibitors for the generation of transgenic pigs
Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.
Piedrahita; Jorge A. (College Station, TX), Bazer; Fuller W. (College Station, TX)
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