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Generated: August 18, 2019

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Patent: 6,274,354

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Summary for Patent: 6,274,354
Title: Methods using cre-lox for production of recombinant adeno-associated viruses
Abstract:Methods for efficient production of recombinant AAV are described. In one aspect, three vectors are introduced into a host cell. A first vector directs expression of cre recombinase, a second vector contains a promoter, a spacer sequence flanked by loxP sites and rep/cap, and a third vector contains a minigene containing a transgene and regulatory sequences flanked by AAV ITRs. In another aspect, the host cell stably or inducibly expresses cre recombinase and two vectors carrying the other elements of the system are introduced into the host cell.
Inventor(s): Wilson; James M. (Gladwyne, PA), Phaneuf; Daniel (Philadelphia, PA)
Assignee: The Trustees of the University of Pennsylvania (Philadelphia, PA)
Application Number:09/242,743
Patent Claims:see list of patent claims

Details for Patent 6,274,354

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04 ➤ Sign Up The Trustees of the University of Pennsylvania (Philadelphia, PA) 2016-09-06 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04 ➤ Sign Up The Trustees of the University of Pennsylvania (Philadelphia, PA) 2016-09-06 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04 ➤ Sign Up The Trustees of the University of Pennsylvania (Philadelphia, PA) 2016-09-06 RX search
Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source

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