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Last Updated: October 22, 2021

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Patent: 6,159,687

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Summary for Patent: 6,159,687
Title: Methods for generating recombined polynucleotides
Abstract:A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, whereby A. extended primers are synthesized by a) denaturing the DNA templates b) annealing primers to the templates, c) extending the said primers by use of a polymerase, d) stop the synthesis, and e) separate the extended primers from the templates, B. a template shift is induced by a) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, or b) repeating steps A.b) to A.e), C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof. Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.
Inventor(s): Vind; Jesper (Lyngby, DK)
Assignee: Novo Nordisk A/S (Bagsvaerd, DK)
Application Number:09/040,697
Patent Claims:see list of patent claims

Details for Patent 6,159,687

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Ferring Pharmaceuticals Inc. NOVAREL chorionic gonadotropin For Injection 017016 1974-01-15 ⤷  Free Forever Trial 2017-03-18
Ferring Pharmaceuticals Inc. NOVAREL chorionic gonadotropin For Injection 017016 1984-12-27 ⤷  Free Forever Trial 2017-03-18
Ferring Pharmaceuticals Inc. NOVAREL chorionic gonadotropin For Injection 017016 1985-02-15 ⤷  Free Forever Trial 2017-03-18
Ferring Pharmaceuticals Inc. NOVAREL chorionic gonadotropin For Injection 017016 1990-02-16 ⤷  Free Forever Trial 2017-03-18
Bel-mar Laboratories, Inc. CHORIONIC GONADOTROPIN chorionic gonadotropin Injection 017054 1974-03-26 ⤷  Free Forever Trial 2017-03-18
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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