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Last Updated: September 18, 2020

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Patent: 5,912,163

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Summary for Patent: 5,912,163
Title: High flow technique for harvesting mammalian cells
Abstract:The invention is based in part on the observation that significant portions of porcine livers appear to remain intact after perfusion by standard methods, suggesting that the perfusion procedures employed do not result in complete enzymatic digestion. Recovery of cells is therefore substantially lower than would be possible if the organs were thoroughly digested. It is found that increased perfusion flow rate, occlusion of at least one major blood vessel leading out of the organ, increased enzymatic digestion time, and vigorous tissue dissociation techniques can be combined to afford a uniquely high yield of viable cells.
Inventor(s): Borland; Kermit M. (Shrewsbury, MA), Chandler; Barbara A. (Lexington, MA), Picton; Dianna Hunt (Bedford, MA), Cain; Shawn P. (North Chelmsford, MA), Deane; Deborah (Orange, MA), Mullon; Claudy J.P. (Framingham, MA)
Assignee: Circe Biomedical, Inc. (Lexington, MA)
Application Number:08/953,541
Patent Claims:see list of patent claims

Details for Patent 5,912,163

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Smith And Nephew SANTYL collagenase OINTMENT;TOPICAL 101995 001 1965-06-04   Start Trial Circe Biomedical, Inc. (Lexington, MA) 2039-03-29 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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