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Last Updated: October 18, 2021

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Patent: 5,876,942

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Summary for Patent: 5,876,942
Title: Process for sexing cow embryos
Abstract:A rapid, highly reproducible and sensitive technique has been successfully developed for sexing the cow embryos, by method of polymerase chain reaction (PCR) against the amelogenin (bAML) genes located on both X- and Y-chromosomes of the Holstein dairy cattle. Results from DNA sequence analysis showed that there was only 45% homology between the intron 5 of AMLX and AMLY genes. Based on these sequences a pair of sex-specific primers, pbAML5XY(+) and pbAML5XY (-),were designed allowing to amplify a single fragment of 476-bp from the female cattle and two fragments of 476-bp and 341-bp from the male ones, respectively. The most important feature is that the precise sensitivity of sex-determination was confirmed to be reached as minimum template as trace amount of genomic DNA content in either a single lymphocyte or a single blastomere isolated from cow embryo at day-6 to day-7. Moreover, neither those of complicated procedures for purifying the DNA prior the PCR nor any extra pair of primers for serving as internal control is thought to be essential and the sex-determination of over hundred embryos can be completed at once within 4hrs.
Inventor(s): Cheng; Winston Teng-Kuei (Taipei, TW), Chen; Chuan-Mu (Tai Chung, TW), Hu; Che-Lin (Taipei, TW), Wang; Chih-Hua (Taipei, TW), Choo; Kong-Bung (Taipei, TW)
Assignee: National Science Council of Republic of China (Taipei, TW)
Application Number:08/899,811
Patent Claims:see list of patent claims

Details for Patent 5,876,942

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 1986-06-04 ⤷  Free Forever Trial National Science Council of Republic of China (Taipei, TW) 2039-02-26 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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