Composition containing collagenase and chymopapain for hydrolyzing connective tissue to isolate cells
Proteolytic enzyme compositions and processes for digesting connective tissue are disclosed. The enzyme compositions include collagenase, which is essentially free of toxins and non-collagen specific components, and chymopapain, which is essentially free of toxins. The enzyme compositions are used for dissociating microvessel cells from connective tissue. Recovered microvessel cells are incorporated into artificial vessel grafts. The enzyme compositions preferably contain an aqueous mixture of collagenase having an activity of about 43 nkat/ml to about 51 nkat/ml, and chymopapain having an activity of about 0.22 nkat/ml to about 0.44 nkat/ml.
Lee; Catherine T. (Laguna Hills, CA), Hornacek; Cynthia (Trabuco cyn, CA), Dinh; Tan T. (Garden Grove, CA)
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